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Filtering method of procoagulant type test tube sample

A filtration method and test tube technology, which is applied in the direction of biological testing, material inspection products, etc., can solve the problems of insufficient sampling volume, affecting the measurement of absorbance, affecting the detection results, etc., and achieve the effects of reducing pollution, facilitating long-term storage, and accurate detection results

Inactive Publication Date: 2010-06-09
CHENGDU RICH SCI IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If it is partially blocked, the sampling volume is not enough, the ratio of serum and reagent will change, so the result will be inaccurate; if there is oil in the serum, the sampling needle will suck the oil into the cuvette, and the oil will be adsorbed on the cuvette, and after a long time The light transmittance of the cuvette will decrease. The automatic biochemical analyzer calculates the result by measuring the absorbance of the solution with a certain wavelength of light. If the light transmittance decreases, it will affect the absorbance measurement, thereby affecting the test result and causing blood sample detection. The result is inaccurate, affecting the doctor's diagnosis of the disease
[0007] After centrifugation of blood samples in procoagulant tubes and ordinary tubes, the red blood cells will dissolve after a certain period of time due to the absence of separation gel for blood cells and serum, resulting in hemolysis of the blood samples, which will affect the test results when the samples are re-examined. Not conducive to long-term preservation of specimens

Method used

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  • Filtering method of procoagulant type test tube sample
  • Filtering method of procoagulant type test tube sample
  • Filtering method of procoagulant type test tube sample

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0030] A method for filtering coagulation-promoting test tube specimens, the steps of which are: a. Using vacuum blood collection technology to collect blood in a coagulation-promoting tube, and a filter screen is arranged inside the coagulation-promoting tube. b. The coagulation-promoting tube is subjected to centrifugal force, and the solution in the coagulation-promoting tube forms zones of different levels. The coagulation-promoting tube is composed of a blood cell layer and a serum layer from bottom to top, and the filter screen goes down the tube wall under the action of centrifugal force. sinks to the layer of blood cells.

[0031] There is a coagulant in the coagulation accelerator, and the filter is a polymer one-way filter. The pore size of the filter is less than 5 microns, and the diameter of blood cells is generally more than 7 microns. The serum is liquid. After centrifugation, the serum layer will pass through the pore size of the filter. The blood cell layer ca...

Embodiment 2

[0033] A method for filtering coagulation-promoting test tube specimens, the steps of which are: a. Using vacuum blood collection technology to collect blood in a coagulation-promoting tube, and a filter screen is arranged inside the coagulation-promoting tube. b. The coagulation-promoting tube is subjected to centrifugal force, and the solution in the coagulation-promoting tube forms zones of different levels. The coagulation-promoting tube is composed of a blood cell layer and a serum layer from bottom to top, and the filter screen goes down the tube wall under the action of centrifugal force. sinks to the layer of blood cells.

[0034] There is a coagulant in the coagulation accelerator, and the filter is a polymer one-way filter. The pore size of the filter is less than 5 microns, and the diameter of blood cells is generally more than 7 microns. The serum is liquid. After centrifugation, the serum layer will pass through the pore size of the filter. The blood cell layer ca...

Embodiment 3

[0036] A method for filtering coagulation-promoting test tube specimens, the steps of which are as follows: a. Using vacuum blood collection technology to collect blood in a common tube, the common tube is provided with a filter screen. b. The ordinary tube is subjected to centrifugal force, and the solution in the ordinary tube forms zones of different levels. The ordinary tube is divided into blood cell layer and serum layer from bottom to top, and the filter screen sinks to the blood cell layer along the tube wall under the action of centrifugal force. superior.

[0037] The filter in the ordinary tube is a polymer one-way filter. The pore size of the filter is less than 5 microns, and the diameter of blood cells is generally above 7 microns. Serum is liquid. After centrifugation, the serum layer will pass through the pore size of the filter, while the blood cell layer cannot pass through the filter. The diameter of the filter is larger than the inner diameter of the coagul...

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Abstract

The invention provides a method for filtering educts after a sample in a procoagulant type test tube is subjected to a centrifugal action. The technical scheme is realized by comprising the following steps of: a. collecting blood into the procoagulant type test tube by using a vacuum blood taking technology, wherein the procoagulant type test tube is internally provided with a filter screen; and b. enabling the procoagulant type test tube to be subjected to the centrifugal action so that a solution in the procoagulant type test tube forms a zone belt with different levels, wherein a blood corpuscle layer and a blood serum layer are positioned in the procoagulant type test tube from bottom to top, and the filter screen sinks on the blood corpuscle layer along a tube wall under the action of centrifugal force. The invention can effectively filter out floating fiber protein filaments and separation gel educts in the blood serum obtained after centrifugation, reduces the pollution of the fiber protein filaments and the separation gel educts on a sampling probe and a colorimetric cuvette, and ensures the detection result of a blood sample to be more accurate.

Description

Technical field: [0001] The invention provides a method for filtering the precipitate in the sample in the coagulation-promoting test tube after centrifugation. Background technique: [0002] At present, the coagulation-promoting test tubes on the market include coagulation-promoting tubes, ordinary tubes and separation rubber tubes, and the three test tubes have the same purpose. The coagulation accelerator is added to the coagulation accelerator tube to accelerate the coagulation of blood samples; there is no coagulant accelerator in the ordinary tube, and the blood sample coagulates relatively slowly; the separation rubber tube is added with coagulant accelerator and separation gel at the same time to quickly separate serum. [0003] After blood is drawn and centrifuged in procoagulant test tubes, there are more or less precipitates of fibrin filaments and separating gel floating in the serum. The main reasons are: [0004] 1. If you use coagulation-promoting tubes or or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/49
Inventor 胡祥
Owner CHENGDU RICH SCI IND
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