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Novel maintenance liquid for mantle pieces of pinctada martensii and preparation method thereof

A technology of pearl oyster and maintenance liquid, applied in the direction of animal cells, etc., can solve the problems of the quality decline of seawater pearls, high yield and low value, etc., and achieve the effect of improving the retention rate and high-quality pearl rate, and improving the quality and output.

Inactive Publication Date: 2010-06-09
GUANGDONG OCEAN UNIVERSITY
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AI Technical Summary

Problems solved by technology

However, due to the lack of scientific research and technological support, the quality of seawater pearls has declined significantly in the past decade, and the phenomenon of "high yield and low value" has appeared. Therefore, it is very urgent to systematically carry out research on seawater pearl breeding technology.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Location: Zhanjiang Xuwen Longxiu State-owned Pearl Field; Time: August 2006. 10,000 shellfish (shell length 6.0-7.5cm) were randomly sampled from the cultured population of Pinctada martensii in Liushagang for the small piece maintenance experiment, and 10,000 shellfish were sampled as the control group at the same time. Use sand filter seawater to dilute the maintenance solution at a volume ratio of 1:4. Soak the clipped mantle pieces with diluent for 30min. Carry out nucleus inserting operation according to conventional technology, brood mother-of-pearl oyster recuperation and sea zone culture. After the recuperation period (the 40th day after seed insertion), the survival rates of pearl oysters in the experimental group and the control group were 90.0±4.71% and 85.3±5.05%, respectively. In June 2008, the rates of high-quality beads in the experimental group and the control group were 57.0±8.9% and 44.9±5.9%, respectively, and the rate of high-quality beads increas...

Embodiment 2

[0012] Location: Zhanjiang Xuwen Guangdong Anhua Group Co., Ltd.; Time: September 2008. 10,000 shellfish (shell length 6.0-7.5cm) were randomly sampled from the cultured population of Pinctada martensii in Liushagang for the small piece maintenance experiment, and 10,000 shellfish were sampled as the control group at the same time. Use sand-filtered seawater to dilute the maintenance solution at a volume ratio of 1:4. Soak the clipped mantle pieces with diluent for 30min. Carry out nucleus inserting operation according to conventional technology, brood mother-of-pearl oyster recuperation and sea zone culture. In May 2009, the high-quality bead rates of the experimental group and the control group were 53.4±7.7% and 43.4±6.5%, respectively, and the high-quality bead rate increased by 25.0%.

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PUM

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Abstract

The invention relates to novel maintenance liquid for mantle pieces of pinctada martensii and a preparation method thereof. The maintenance liquid consists of NaCl, KCl, MgCl2, CaCl2 and prednisone acetate. The preparation method comprises the steps of: weighing and dissolving the NaCl, the KCl, the MgCl2, the CaCl2 and the prednisone acetate into double distilled water sequentially; and regulating a PH value to between 7.8 and 8.0 by adopting NaHCO3. The maintenance liquid has the advantages that the process is simple, the cost is low, the nucleus retaining rate and the high-quality pearl rate of pearl-culturing mollusk of the pinctada martensii can be improved obviously, and the quality and yield of pearls are improved.

Description

technical field [0001] The invention relates to a novel pearlite martensii cell sheet maintenance solution and a preparation method thereof. Background technique [0002] In my country, Pinctada martensii is mainly distributed in the coastal areas of Guangdong, Guangxi and Hainan provinces. Historically, pearls cultivated from Pinctada martensii are known as "South Pearls", and are well-known at home and abroad for their roundness, dignified, crystal clear, smooth and delicate, and long-lasting color. Since the 1960s, seawater pearl culture of Pinctada martensii has developed rapidly in my country, and has become a pillar industry for marine economic development in Guangdong, Guangxi and some coastal areas of Hainan. At present, my country's seawater pearl cultivation area is nearly 200,000 mu, with an annual output of about 20 tons of pearls, and the output value of unprocessed primary products is about 200 million yuan. However, due to the lack of scientific research and...

Claims

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Application Information

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IPC IPC(8): C12N5/07
Inventor 杜晓东邓陈茂符韶邓岳文梁飞龙
Owner GUANGDONG OCEAN UNIVERSITY
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