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Alpha-glucosidase inhibitor and preparation method thereof

A glucosidase and inhibitor technology, which is applied in the field of α-glucosidase inhibitor and its microbial fermentation preparation, can solve the problems of difficult industrialization and low extraction efficiency of traditional Chinese medicine, and achieves low production cost, improved production efficiency, and improved operation. Easy to implement effects

Inactive Publication Date: 2010-06-09
GUANGXI NANNING ZHITIAN BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a kind of α-glucosidase inhibitor and preparation method thereof, this method can solve the problem that the extraction efficiency of traditional Chinese medicine is low, is difficult to realize the problem of industrialization

Method used

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  • Alpha-glucosidase inhibitor and preparation method thereof
  • Alpha-glucosidase inhibitor and preparation method thereof
  • Alpha-glucosidase inhibitor and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Activation of strains: transfer the activated strains to slant medium to cultivate and accumulate a large number of spores for mass preparation of seeds.

[0028] The weight composition of the medium is: soluble starch 2.0%, KNO 3 0.1%, NaCl 0.01%, K 2 HPO 4 0.01%, MgSO 4 ·7H 2 O 0.01%, FeSO 4 0.001%, agar powder 1.8%, the balance is water, pH 7.0, culture temperature 28 ℃, culture time 7d.

[0029] (2) Seed cultivation

[0030] Use an inoculation loop to pick a ring of spores from the slant and connect them to the seed medium. After inoculation, culture the shake flasks at 26°C, 28°C, 30°C, 32°C, and 34°C at 160r / min. 24h, three parallel experiments were set for each temperature.

[0031] The weight composition of the medium is: 2% corn flour, 1.0% soluble starch, KNO 3 0.5%, NaCl 0.05%, K 2 HPO 4 0.05%, MgSO 4 ·7H 2 0.05%, FeSO 4 0.001%, the balance is water.

[0032] pH 7.2, the filling volume is 50ml / 250ml.

[0033] (3) Fermentation culture ...

Embodiment 2

[0043] (1) Activation of bacteria

[0044] With embodiment 1.

[0045] (2) Seed cultivation

[0046]Prepare the seed culture medium, the initial pH of each seed culture medium is 6.0, 7.0, 8.0, 9.0, 10.0 respectively, after sterilization and cooling. Use an inoculation loop to pick a ring of spores from the slant and inoculate them into the seed medium, culture the shake flasks at 30°C and 160r / min for 24 hours, and set up three parallel experiments for each pH value.

[0047] The weight composition of the culture medium is the same as in Example 1.

[0048] (3) Fermentation culture

[0049] Fermentation shake flask is 250ml, filled with 50ml of fermentation medium, the initial pH value of the fermentation medium is 6.0, 7.0, 8.0, 9.0, 10.0 respectively After sterilization, when transferring, first mix three parallel seed shake flasks in Together, then use a 5ml pipette gun to draw 5ml of seeds and transfer them to fermentation shake flasks, and inoculate 3 shake flasks wi...

Embodiment 3

[0058] (1) Strain activation:

[0059] With embodiment 1.

[0060] (2) Seed cultivation

[0061] Prepare the seed medium, the initial pH value is 9.0, the liquid volume of the seed medium is 50ml / 250ml, pick a ring of spores from the spores on the slope with an inoculation loop and connect them to the seed medium, and place the shaker flasks at 30°C , 160r / min culture, 24h, set up three parallel experiments.

[0062] The weight composition of the culture medium is the same as in Example 1.

[0063] (3) Fermentation culture

[0064] Prepare the fermentation medium, the initial pH is 9.0, and the filling volume is 20ml / 250ml, 30ml / 250ml, 40ml / 250ml, 50ml / 250ml, 60ml / 250ml, 70ml / 250ml, 80ml / 250ml, 90ml / 250ml, 100ml / 250ml, after sterilization, when transferring, first mix three parallel seed shake flasks together, then use a 5ml pipette gun to transfer 5ml of seeds to the fermentation shake flasks, inoculate 3 shake flasks each, and place at 30 Cultivate under the condition ...

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Abstract

The invention relates to an alpha-glucosidase inhibitor and a preparation method thereof. The preparation method comprises the following steps: culturing and activating a strain; culturing seeds; inoculating the stain into a shake flask for fermentation culture; collecting fermentation liquor; and performing separation and purification to obtain the alpha-glucosidase inhibitor. In the invention, the alpha-glucosidase inhibitor is obtained by microbial fermentation and can be used for developing hypoglycemic medicaments and provide a new tool for further research on active site of enzymes such as the alpha-glucosidase.

Description

technical field [0001] The invention relates to an alpha-glucosidase inhibitor and a preparation method thereof by microbial fermentation. Background technique [0002] Diabetes, obesity, and hyperlipidemia are prevalent and continue to increase in the worldwide population. Diabetes mellitus is one of the most chronic diseases in the general population, developing with increasing obesity and age. Although there are drugs developed for the treatment of diabetic syndrome, the most challenging goal in the treatment of diabetic patients today remains to bring blood glucose levels as close to normal as possible. In addition, postprandial hyperglycemia and hyperinsulinemia are independent risk factors for diabetic macrovascular complications. "Diabetes Medicine" reported that α-glucosidase inhibitors exist in the α-glucosidase of small intestinal microvilli, thereby inhibiting the rapid increase after eating and the increase in insulin concentration caused by it, because it can ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K4/04A61K38/03A61P3/10
Inventor 梁智群陈桂光石君连张云开丁苏
Owner GUANGXI NANNING ZHITIAN BIOTECH
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