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Tissue culture rapid propagation method for crocus

A tissue culture, saffron technology, applied in horticultural methods, botanical equipment and methods, plant cells, etc., can solve the problems of lack of germplasm resources, bulb degradation, and low crocus yield.

Active Publication Date: 2010-06-09
上海上药华宇药业有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Because crocus cannot reproduce sexually, it can only reproduce asexually through bulbs, and its bulbs degenerate seriously under cultivation conditions, resulting in a serious shortage of germplasm resources.
Since the introduction of the species in China, the yield of cultivated saffron has been low, which is far from meeting people's needs.

Method used

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  • Tissue culture rapid propagation method for crocus
  • Tissue culture rapid propagation method for crocus
  • Tissue culture rapid propagation method for crocus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) Take the corms of the current year's crocus as explants, rinse the corms in tap water, remove the skin film, and place them on an ultra-clean workbench for disinfection and inoculation. Rinse in 70% alcohol for 30s, then use 0.1% HgCl 2 Disinfect for 8-10 minutes, and finally rinse 5 times with sterile water to thoroughly wash away residual HgCl 2 .

[0040] (2) Cut the sterilized corm into 1cm 3 Size, inoculated on MS+0.5mg / L NAA+5.0mg / L6-BA medium, and cultured for 20 days under dark conditions (24 hours without light) to harvest callus. The callus can be subcultured for a long time under this condition without browning.

[0041](3) The callus was transferred to MS+0.5mg / L NAA+2.0mg / L 6-BA medium, and cultured at 20°C under dark conditions after 20 days, a large number of clustered shoots could be induced.

[0042] (4) Cut the clustered buds into individual buds and transfer them to MS+0.5mg / L NAA+2.0mg / L 6-BA medium, culture at 20°C under dark conditions (24 ...

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Abstract

The invention discloses a tissue culture rapid propagation method for crocus. The method comprises the steps of explant sterilization, callus induction, clustered shoot induction culture, aventitious shoot subculture, corm induction culture and the like so as to finally obtain the induced corm. The invention adopts the tissue culture method to perform the callus induction and corm induction culture of crocus, establish the reproductive system of crocus superior varieties and perform industrial seedling production of seedlings and can fast propagate the superior varieties in large.

Description

technical field [0001] The invention relates to a plant tissue culture and propagation method, in particular to a tissue culture rapid propagation method of saffron. Background technique [0002] Saffron (Crocus sativus L.), also known as saffron and saffron, is a perennial herb of the genus Crocus in the family Iridaceae. It is originally produced in southern European countries and Iran, and then it was transferred to Tibet through India and then introduced to China. It was successfully introduced and cultivated, so it is also called saffron or saffron. Because saffron is only used as medicine on the stigma, the yield is extremely low, and it takes time and effort to harvest; at the same time, the source of provenance is limited, the suitable cultivation area is limited, and the conditions are harsh, which makes it expensive. It is listed as a rare and precious Chinese medicinal material and is known as the "Plant Gold". The pistil stigma of saffron is a precious traditio...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 秦路平汪洋杨弘宋嬿
Owner 上海上药华宇药业有限公司
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