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Technology for producing alpha-cyclodextrins by biological method

A production process and technology of cyclodextrin, which is applied in the field of biological production of α-cyclodextrin, can solve the problem of lack of α-cyclodextrin production process, low solubility of β-cyclodextrin, lack of good strains, etc. problem, to achieve the effect of simple removal process, low production cost and less impurities

Active Publication Date: 2010-05-26
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for efficient production of alpha - CGT by converting sucrose into beta (α)-glutathione peroxidases). It also simplifies the manufacturing processes while maintaining their quality level. Additionally, adding an appropriate concentration of sugar or other substances helps improve its properties during processing. Overall, these improvements make the process more effective at generating alpha -CGT products compared to previous methods.

Problems solved by technology

This patented technical problem addressed in this patents relates to finding an effective way to produce α -cyclic dextrumbers without generating waste materials during purification processes like filtrations. Current techniques involve either creation of stable forms through various means including chemical synthesis or bioconversion, resulting in increased costs compared to other ways of making them commercially available.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Raw material pretreatment:

[0029] The potato starch slurry is adjusted according to the concentration of 10%, and stirred for 5-15 minutes under the condition of 50-90 DEG C, so that the starch granules are fully swollen.

[0030] Enzymatic production process:

[0031] After pretreatment, set the temperature to 30°C, adjust the pH to 5.0, add α-CGTase at a rate of 10 units per gram of starch, and then add 5% decanyl alcohol by reaction volume, and fully react for 8-10 hours.

[0032] α-CGT extraction process:

[0033]Filter the reaction solution directly, wash the filter cake with distilled water 2-3 times, collect the filter cake, including the complex precipitate formed by n-decyl alcohol and α-CGT and unreacted starch; redissolve the filter cake, and then use Steam distillation to remove n-decyl alcohol, after the distillation is complete, filter the distillate to remove unreacted starch to obtain an aqueous solution of α-CGT; evaporate and concentrate the aqueou...

Embodiment 2

[0038] Raw material pretreatment:

[0039] Same as Example 1

[0040] Enzymatic production process:

[0041] After pretreatment, set the temperature to 40°C, adjust the pH to 5.0, add α-CGTase at a rate of 10 units per gram of starch, and then add 5% decanyl alcohol by reaction volume, and fully react for 8-10 hours.

[0042] α-CGT extraction process:

[0043] Same as Example 1

[0044] The results are shown in Table 1. The total conversion rate of starch is as high as 65%, the proportion of α-CGT in the product is 75%, and the proportion of β-CGT is 25%.

Embodiment 3

[0046] Raw material pretreatment:

[0047] Same as Example 1

[0048] Enzymatic production process:

[0049] After pretreatment, set the temperature to 50°C, adjust the pH to 5.0, add α-CGTase at a rate of 10 units per gram of starch, and then add 5% decanyl alcohol to react fully for 8-10 hours.

[0050] α-CGT extraction process:

[0051] Same as Example 1

[0052] The results are shown in Table 1. The total conversion rate of starch is as high as 63%, the proportion of α-CGT in the product is 72%, and the proportion of β-CGT is 28%.

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Abstract

The invention discloses technology for producing alpha-cyclodextrins (alpha-CGT) by a biological method, belonging to the technical field of cyclodextrins production. The alpha-CGT is produced by adopting alpha-cyclodextrins glycosyltransferase (alpha-CGTase) derived from paenibacillus macerans (China Center for Type Culture Collection of Microbe, CCTCCM 208063). By applying the technology to produce alpha-CGT, the total conversion of starch is as high as 71%, and in the product, alpha-CGT accounts for 80% and beta-CGT accounts for 20%. The technology has the advantages of simple flow, short production cycle, high conversion and high purity of the obtained alpha-CGT, etc.

Description

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Claims

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Application Information

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Owner JIANGNAN UNIV
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