Organophosphorus pesticide degrading bacterium and method for producing microbial inoculum thereof

Abstract

The invention relates to bacillus capable of efficiently degrading an organophosphorus pesticide and separated from rape phyllospheres, and provides a method for preparing a microbial inoculum thereof, which belong to the field of biotechnology. The bacillus is identified as bacillus subtilis, which is preserved in the China General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms, wherein the preservation No. is CGMCC No. 3374; the 16S rDNA gene sequence of the bacillus strain is submitted to a GenBank database; and the accession number is GU086422. The method for producing the microbial inoculum comprises the following 5 steps of: the preparation of a culture medium, the activation of a bacterium strain, the preparation of a liquid seed, the liquid state fermentation and the preparation of the microbial inoculum, wherein the fermentation culture medium takes molasses as a main raw material. Because the organophosphorus pesticide degrading microbial inoculum prepared by the technical scheme of the invention can efficiently degrade the organophosphorus pesticide residue on plant leaves in situ, the microbial inoculum can guarantee the safety of foods and protect the ecological environment.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a bacillus isolated from the phyllosphere of rapeseed and capable of efficiently degrading organophosphorus pesticides, and a production method thereof. technical background [0002] Organophosphorus pesticides are still the most used category of pesticides because of their many varieties, high efficacy, and broad-spectrum prevention and control. They have played a pivotal role in agricultural production. More than half of the total production and usage. However, organophosphorus pesticides are highly toxic. After long-term and large-scale use, their residues on crops will cause food safety problems and affect the export of agricultural products in my country. Moreover, scattered pesticides will also pollute water and soil. [0003] The bioremediation method using microorganisms to degrade organophosphorus pesticide residues has the advantages of low cost, non-toxicity, and no seconda...

AI Technical Summary

Problems solved by technology

For pesticide-degrading microorganisms isolated from soil, sludge and other sources, they may not be able to adapt to the harsh environment of plant foliage, and their degradation efficiency will often be significantly affected

So far, no literature reports have been found on the isolation of Bacillus degrading organophosphorus pesticides from the phyllosphere of rapeseed and the production method of the bacterial agent