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Image processing based fast automatic focusing method of microscope

An automatic focus and image processing technology, applied in microscopes, optics, instruments, etc., can solve the problems of slow automatic focus and inaccurate focus

Inactive Publication Date: 2010-05-12
常州超媒体与感知技术研究所有限公司 +1
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Problems solved by technology

[0008] In order to overcome the deficiencies of slow auto-focus and inaccurate focus of existing microscopes, the present invention provides a rapid auto-focus method for microscopes based on image processing

Method used

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  • Image processing based fast automatic focusing method of microscope
  • Image processing based fast automatic focusing method of microscope
  • Image processing based fast automatic focusing method of microscope

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Embodiment Construction

[0050] The present invention will now be described in further detail in conjunction with the accompanying drawings and preferred embodiments. These drawings are all simplified schematic diagrams, which only illustrate the basic structure of the present invention in a schematic manner, so they only show the configurations related to the present invention.

[0051] Such as figure 2 It is a flowchart of the rapid automatic focusing algorithm of the microscope proposed by the present invention, a rapid automatic focusing method of the microscope based on image processing, which divides the automatic focusing process into two stages of coarse focusing and fine focusing, and uses different focusing evaluation functions in different stages And the extreme value search strategy, in the coarse focusing stage, the gray difference evaluation function and the fast climbing backtracking search algorithm are used, and the decision factor is introduced to prevent the search algorithm from f...

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Abstract

The invention relates to the technical field of automatic focusing of microscopes, in particular to an image processing based fast automatic focusing method of a microscope. In the method, the automatic focusing process is divided into a coarse focusing stage and a fine focusing stage; different focusing evaluation functions and different extremum searching strategies are adopted on different stages, specifically, on the coarse focusing stage, the gray scale different evaluation function and a fast hill-climbing backtracking search algorithm are adopted, and decision-making factors are introduced to prevent the search algorithm from falling into local extrema; and on the fine focusing stage, the Laplace operator based evaluation function and the small-step searching strategy are adopted. The method provided by the invention better solves the problem that the traditional algorithm is slow in focusing speed, easily falls into local extrema and is not high in focusing accuracy, and has strong real-time property and high reliability and practicability.

Description

technical field [0001] The invention relates to the technical field of microscope autofocus, in particular to a rapid autofocus method for microscopes based on image processing. Background technique [0002] The traditional microscope focusing method is to use manual adjustment. This method completely relies on human naked eye observation and manual adjustment, and the focusing efficiency is very low. In recent years, automatic focusing methods based on image processing technology have been gradually applied to the microscope focusing process, but at present, these methods still have the problems of slow focusing speed, easy to fall into local extremum, and low focusing accuracy. [0003] Autofocus is a key technology in fully automatic image acquisition, widely used in digital cameras, photomicrographs, fine processing, optical measurement equipment, robot vision navigation and other fields. According to its focusing principle, automatic focus can be divided into two types...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G02B21/24
Inventor 刘云辉刘顺翟永平周东翔蔡宣平
Owner 常州超媒体与感知技术研究所有限公司
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