Uridine diphosphate xylose isomerase, coding gene thereof and use thereof

A technology of uridine diphosphate xylose and uridine diphosphate arabinose, applied in the direction of isomerase, application, genetic engineering, etc., can solve the problem of loss of stomatal opening and closing function of isolated commelina leaves, and achieve a major economic Value and application prospects, fiber length increase, effect of improving quality and yield

Inactive Publication Date: 2010-04-28
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In 2003, Jones et al. used arabinanase to hydrolyze pectin RG I to lose the opening and closing function of stomata in isolated Commelina leaves

Method used

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  • Uridine diphosphate xylose isomerase, coding gene thereof and use thereof
  • Uridine diphosphate xylose isomerase, coding gene thereof and use thereof
  • Uridine diphosphate xylose isomerase, coding gene thereof and use thereof

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Embodiment 1

[0047] Embodiment 1, cloning of uridine diphosphate xylose isomerase coding gene

[0048] 1. Cloning of GhUXE1 gene

[0049] Select wild-type upland cotton variety Xuzhou 142 (WT) (National Mid-term Cotton Seed Bank of Cotton Research Institute, Chinese Academy of Agricultural Sciences) fibers 10 days after flowering, extract total RNA, and extract total RNA according to the RNA gentsTotal RNA Isolation System kit of Promega Company conduct. Take 5g of total RNA and reverse transcribe to obtain one strand of cDNA. The reaction system is as follows:

[0050] Total RNA 10 μL (5 μg), Oligo (dT) (20 μmol / L) 1.5 μL, 10× buffer 2.5 μL, dNTPmix (2.5 mmol / L) 2 μL, sterile water 8 μL.

[0051] Add 1 μL (200U) SuperScript to the reaction system after bathing in water at 42°C for 1 min TM II RT, mixed gently, incubated at 42°C for 50min; bathed in water at 70°C for 15min, terminated the reaction; obtained the first strand of cDNA.

[0052] Primers were designed for PCR amplification,...

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Abstract

The invention discloses a uridine diphosphate xylose isomerase, a coding gene thereof and use thereof. The uridine diphosphate xylose isomerase is a protein a or a protein b, wherein the protein a has an amino acid sequence represented by the No.2 sequence in a sequence table; and the protein b is derived from the protein a by substituting and / or losing and or adding one or several amino acids in the amino acid sequence represented by the No.2 sequence in the sequence table and is related to the synthesis of the uridine diphosphate arabinose. The invention also discloses a coding gene for coding the protein. The coding gene of the uridine diphosphate xylose isomerase can be introduced into cotton to lengthen cotton fibers and improve the quality and yield of cotton fibers. The uridine diphosphate xylose isomerase and the coding gene thereof have great economic values and application prospects.

Description

technical field [0001] The invention relates to uridine diphosphate xylose isomerase, its coding gene and application. Background technique [0002] Cotton fiber is a unicellular structure differentiated from the outer skin cells of the ovule. Cotton fiber is an important raw material for the textile industry and has important economic value. Fiber quality determines its final length and strength. At the same time, cotton fiber cells are also an ideal system for studying important biological phenomena such as cell elongation, differentiation and cell wall synthesis. Therefore, the study of fiber elongation has important economic value and theoretical significance. [0003] The development process of cotton fiber cells is a process of supernormal cell elongation and supernormal cell wall thickening. The length of upland cotton is about 3.0cm, and the diameter of the upland cotton cell wall is 11-22μm, that is to say, the aspect ratio is 1000-3000. Cotton fiber cell forma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/90C12N15/61C12N15/63C12N5/10C12N1/00C12P19/30C12N15/82C12N15/11
Inventor 朱玉贤庞朝友逄宇宋文强王慧靳翔秦咏梅
Owner PEKING UNIV
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