Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bacterial strain capable of degrading petroleum hydrocarbon and application thereof

A technology for petroleum hydrocarbons and bacterial agents, which is applied in the field of bacterial strains for degrading petroleum hydrocarbons, can solve the problems of harmful chemical extractants, limited widespread use, expensive equipment costs, etc., and achieves high activity, reduces complexity and safety, and accelerates progress. Effect

Active Publication Date: 2010-04-07
PEKING UNIV
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chemical extraction agents are mostly harmful to aquatic ecological species, and their side effects are more serious than the direct economic losses caused by the flooding of oil pollution. Therefore, many countries have banned the use of this method
The physical method is to absorb and collect oil pollution through some equipment or additives. Many types of such devices have been developed, and the removal effect is good, but the expensive equipment costs limit its wide use, and the effect is low in cold seasons

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacterial strain capable of degrading petroleum hydrocarbon and application thereof
  • Bacterial strain capable of degrading petroleum hydrocarbon and application thereof
  • Bacterial strain capable of degrading petroleum hydrocarbon and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1, isolation and identification of bacterial strain

[0044] 1. Separation

[0045] Soil source: Select contaminated soil samples from Shandong Oilfield. The oilfield here is currently the main oil production area in my country, and its oil pollution is also the most serious. Therefore, the content and degradation ability of oilophilic bacteria in the soil should also be very strong.

[0046] Enrichment and screening of oleophilic bacteria by plate culture: Take 5.0g of petroleum-contaminated soil samples and put them into a 250ml Erlenmeyer flask containing 100ml of MSM medium, and place them at 30°C, 150r min -1 Cultivate in a shaker under certain conditions for 4 days; then take a certain amount of supernatant liquid into a 250ml Erlenmeyer flask containing 100ml of fresh medium, 30°C, 160r min -1 Cultivate in shaker under the condition for 7d; so repeat 3 times. Dip the enriched culture solution with an inoculating loop and streak on the plate; after mu...

Embodiment 2

[0105] Embodiment 2, the cultivation of bacterial strain and the preparation of solid microbial agent

[0106] In many oil sludge bioremediation, the addition of bacteria is a very troublesome step, because the bacteria liquid is added to the oil sludge, but the biggest defect of the bacteria liquid is that it is not conducive to transfer, that is, it is not easy to safely and quickly remove the bacteria from the experiment The room is transferred to the actual site.

[0107] 1. Composition and preparation of bacterial agent I

[0108] 1. Composition:

[0109] Bacteria I is made of soybean flour, WTS bacteria, (NH 4 ) 2 HPO 4 and MgSO 4 Mixed composition, wherein, soybean flour, (NH 4 ) 2 HPO 4 , MgSO 4 The ratio of bacteria and bacteria is 15g soybean flour: 0.9g (NH 4 ) 2 HPO 4 : 0.02g MgSO 4 : 15×10 9 CFU bacteria.

[0110] 2. Preparation steps of bacterial agent

[0111] (1) Culture of bacteria

[0112] Cultivate WTS bacteria with LB medium at 30°C and 130...

Embodiment 3

[0133] Embodiment 3, the application of bacterial strain

[0134] 1. Degradation of diesel by strain WTS

[0135] Infrared spectrophotometry (GB / T16488-1996) was used to measure the degradation rate of the strain.

[0136] The specific steps are as follows: Take a 50ml triangular flask, add 20ml MSM culture solution, 0.2ml sterilized diesel oil, and 1ml concentration of about 10 9 CFU / ml fresh bacterial solution, 30°C, 150r min -1 Cultivate under conditions for 10 days, and measure the diesel content in the Erlenmeyer flask (ie in the reaction system) every two days. During the measurement, the liquid in the Erlenmeyer flask was put into a centrifuge, and centrifuged at a speed of 10000r / min for 10min. Pour all the supernatant into a 125ml separatory funnel. Add 3 drops of 1:1 sulfuric acid for acidification, a small amount of NaCl as a demulsifier, and 5ml of refined CCl 4 Clean the original triangular flask with the solution, pour it into a separatory funnel, and add ab...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a bacterial strain capable of degrading petroleum hydrocarbon and application thereof, wherein, the bacterial strain is Citrobacter sp. WTS (CGMCC No.3253) highly capable of degrading petroleum hydrocarbon. The bacterial strain, bacterial agent and application method thereof in the invention provide theoretical basis for the bioremediation of oil-polluted soil in China, have great significance to the environmental protection of oil-field areas in China and the remediation of pollution caused by leakage in the oil transportation, and accelerate the progress of bioremediation of oil pollution, therefore, the invention is of great economic significance.

Description

technical field [0001] The invention relates to a bacterial strain for degrading petroleum hydrocarbons and its application. Background technique [0002] Petroleum is a viscous, flammable mixture that occurs naturally beneath the surface of the earth. In terms of composition, it is a complex mixture containing various hydrocarbons (normal alkanes, branched alkanes, aromatics, alicyclic hydrocarbons) and a small amount of other organic compounds (sulfides, nitrides, naphthenic acids). A typical petroleum sample contains as many as 200-300 different hydrocarbon compounds, with molecular weights ranging from 16 to around 1000. Its physical states include gases, volatile liquids, high-boiling liquids, and solids. The crude oil used in general oil fields is divided into paraffin base (alkane > 70%); naphthenic base (cycloalkane > 60%); intermediate base (alkane, naphthene, aromatic hydrocarbon content is close) and asphalt base (asphaltenes > 60%) %). [0003] With ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20A62D3/02B09C1/10C12R1/01A62D101/20
Inventor 黄艺汪杰礼晓
Owner PEKING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com