Microfluidic chip for integrated solid phase micro extraction and detection method

Abstract

The invention relates to a microfluidic chip for integrated solid phase micro extraction, which comprises a solid phase micro extraction passage and a separation passage arranged crosswise. An extraction section is arranged between a first end of the extraction passage and a cross point (junction of the extraction passage and the separation passage), and porous microspheres serving as a solid phase for solid phase micro extraction are filled in the extraction section. In one specific embodiment, the porous microspheres are inorganic granules of which surfaces are wrapped with a layer of organic adsorption film. In the other more specific embodiment, the porous microspheres are carbon dioxide granules. The microfluidic chip of the invention is convenient to replace extraction materials, and full process analysis of sample concentration, separation and detection can be carried out on one microfluidic chip.

Description

technical field [0001] The invention belongs to the field of sample analysis and detection, and more specifically relates to the design of a microfluidic chip with a sample concentration function and its application method in the field of analysis and detection. Background technique [0002] Sample pretreatment has always been the most critical and time-consuming link in the sample analysis process. Traditional solvent extraction is one of the common means of sample pretreatment, but it has the disadvantages of using toxic organic solvents and cumbersome operation. For this reason, people have developed a solvent-free extraction technology, but the equipment is complicated and the test conditions are harsh, which is not suitable for wide application. In 1989, Pawlizyn et al. developed solid-phase microextraction technology on the basis of solid-phase extraction. This technology realizes the extraction and concentration of samples in a quartz fiber. The concentrated samples ...

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Problems solved by technology

However, the solid-phase microextraction technology also has the following disadvantages: the quartz fiber is easily broken during use; it is easy to cause coating contamination when extracting complex matrix samples; there are not many types of coatings, which limits its application range; commercial fibers are expensive , it is not easy to achieve popularization and popularization

But these methods all have certain defect, and one is that the operation of forming adsorption film or porous monolithic column in the channel of micro size is inconvenient, can't control and check the formation quality of solid-phase adsorption medium; The second is that this adsorption film or The monolithic column aggregated in the channel is fixed in the solid phase microextraction channel of the chip. Once the adsorption medium fails, the whole chip can no longer be used

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