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Novel method for increasing yield of epsilon-poly-L-lysine

A technology for lysine and lysine fermentation, which is applied in the field of fermentation engineering and can solve the problems of no flow addition of L-lysine and the like

Active Publication Date: 2014-03-26
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] L-lysine is the direct precursor of ε-poly-L-lysine, but in the existing fermentation process of ε-poly-L-lysine, there is no report about adding L-lysine

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] What embodiment 1 described is existing production technology;

Embodiment 2

[0024] Embodiment 2, embodiment 3 and embodiment 4 are innovative processes compared with embodiment 1.

[0025] Example 1:

[0026] In a 5L fermenter with 2.7L medium, inoculate 300mL of the seed culture solution of Streptomyces amylase chromogenes or Streptomyces albicans, cultivate at 30°C, when the DO drops to 30%, it will be automatically controlled to 30%, and cultured in aerobic conditions for 120h , the air flow rate is 4.0~6.0L / min. When the pH drops to about 6.0, add ammonia (25-30%) to maintain the pH at 6.0, and when the residual sugar concentration in the fermentation broth drops to 10g / L, add glucose (400g / L) and (NH 4 ) 2 SO 4 (80g / L) to make the residual sugar concentration reach 13g / L. At the same time, the pH is no longer controlled, let it drop to 4.0 naturally and maintain it around 4.0.

[0027] After culturing for 120 hours, the fermentation was terminated, and the highest accumulated ε-poly-L-lysine in the fermentation broth was 10.7 g / L. Centrifug...

Embodiment 3

[0032] In a 30L fermenter with 16.2L of medium, inoculate 1.8L of the secondary shaker flask seed culture solution of amylase chromogenic Streptomyces or albicans, cultivate at 30°C, and automatically control it to 30 when the DO drops to 30%. %, aerobic culture for 120h, the air flow rate is 4.0 ~ 6.0L / min. When the pH drops to about 6.0, add ammonia (25-30%) to maintain the pH at 6.0, and when the residual sugar concentration in the fermentation broth drops to 10g / L, add glucose (400g / L) and (NH 4 ) 2 SO 4 (80g / L) makes the residual sugar concentration reach 13g / L, and in the fermented liquid, flow adds L-lysine (10g / L). At the same time, the pH is no longer controlled, let it drop to 4.0 naturally and maintain it around 4.0.

[0033]After culturing for 120 hours, the fermentation was terminated, and the highest accumulated ε-poly-L-lysine in the fermentation broth was 14.6 g / L. Centrifuge to remove bacteria and some solids, use D152 macroporous weakly acidic cation exch...

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Abstract

The invention relates to a novel method for increasing the yield of epsilon-poly-L-lysine, comprising the following steps: adopting Streptomyces diastatochromogenes or Streptomyces albulus which has resistance to S-(2 amino ethyl)-L- cysteine (called AEC for short), glycin and sulfaguanidine), and carrying out the fermentation production of the epsilon-poly-L-lysine through adding mixed solution of L-lysine, glucose and (NH4)2SO4 in a fed-match mode. The novel method can increase the yield by 25-50 percent than the process of with out the fed batch of L-lysine. The invention changes the method of the original production process, obviously increases the yield of the epsilon-poly-L-lysine, lowers the cost, and can be used for industrial scale fermentation.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to a new method for improving the production of ε-poly-L-lysine by a strain of amylase Streptomyces diastatochromogenes or Streptomyces albulus. The new method It involves feeding L-lysine in the fermentation process. Background technique [0002] ε-poly-L-lysine is a lysine homopolymer containing 25 to 30 residues, it is easily soluble in water, but insoluble in organic solvents such as ethyl acetate, ethanol, ether; its heat stability It has high antibacterial activity, and it still has antibacterial activity after heating at 120°C for 10 minutes. ε-poly-L-lysine is a polypeptide with antibacterial effect. It has a wide antibacterial spectrum. It is effective against Gram-positive bacteria, Gram-negative bacteria, molds and yeasts under neutral and slightly acidic conditions. Bacteria have inhibitory effect, and it also has inhibitory effect on heat-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/02C12R1/47C12R1/525
Inventor 贾士儒莫治文谭之磊王国良范宝庆王甜李琳周斌
Owner TIANJIN UNIV OF SCI & TECH
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