TLC identification method for Naoxinqing tablet and persimmon leaf extract

Abstract

The invention discloses a TLC identification method for Naoxinqing tablet and persimmon leaf extract. The method includes the following steps: a) a prepared test article is ground and porphyrized, methanol is added, and the mixture obtained is processed by ultrasonication and then is filtered; filter liquor is dried by distillation, water is added to residuals, the mixture obtained is thoroughly stirred so as to dissolve the residuals, filtration is carried out, hydrochloric acid is added to the filter liquor, absolute ether is used for shaking extraction, and an aether layer is separated to be obtained and then is dried by volatilization; the methanol is added for dissolving the residuals, and first test article solution can be obtained; b) proportioned with the chemical ingredients, a persimmon leaf is processed by the operations identical with the step a), and obtained liquor is used as a first reference drug solution; c) a protocatechuic acid reference substance is prepared and then is added with and dissolved in the methanol, and obtained liquor is used as a first reference substance solution; and d) the first test article solution, the first reference drug solution and the first reference substance solution are processed by the TLC analysis. The test article is not the Naoxinqing tablet or the persimmon leaf extract, providing that in the chromatogram of the test article,spots with the same color can not be displayed on corresponding positions on the chromatograms of the reference drug and the reference substance.

Description

technical field [0001] The invention relates to a TLC identification method for Naoxinqing tablet and persimmon leaf extract, belonging to the field of drug identification. Background technique [0002] Naoxinqing Tablet is a traditional Chinese medicine preparation made of persimmon leaf extract after the persimmon leaves are extracted with water and ethanol, and then extracted with ethyl acetate, and then added with auxiliary materials. Whampoa Traditional Chinese Medicine Co., Ltd.'s exclusive protected species of traditional Chinese medicine, which is recorded on page 294 of the twentieth volume of traditional Chinese medicine prescriptions in the "Drug Standards of the Ministry of Health of the People's Republic of China". There are two specifications of Naoxinqing tablet, among which the approval number of specification (1) [each tablet weighs 0.41g (grams)] is Z44021765, and the implementation standard is WS 3 -B-3974-98, specification (2) [each piece weighs 0.41g (e...

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Problems solved by technology

[0005] In the research, the inventor found that in the original standard identification method, when using the three organic acids "furoic acid, protocatechuic acid and succinic acid" for identification, an unknown compound with a relatively high content in Naoxinqing Tablets was mistakenly identified as (possibly malic acid) mistaken for succinic acid and poorly reproducible identification of furoic acid and protocatechuic acid

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