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Test strip for quickly detecting ureaplasma urealyticum antibodies in saliva

A technology for detecting Ureaplasma urealyticum and test strips, which is applied in the field of saliva rapid detection test strips for Ureaplasma urealyticum antibodies. highly specific effect

Inactive Publication Date: 2010-02-24
杭州艾力康医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The invention provides a test strip for rapid detection of ureaplasma urealyticum antibody saliva. The test strip can use saliva as a sample, which solves the problem that the existing test strip is not suitable for on-site detection and the detection cost is high

Method used

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  • Test strip for quickly detecting ureaplasma urealyticum antibodies in saliva

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Preparation of colloidal gold pads

[0025] Prepare a colloidal gold solution with a diameter of 15nm by the chloroauric acid-trisodium citrate reduction method. After the preparation is completed, take 100ml of the colloidal gold solution in a beaker and use 0.1M K 2 CO 3 Adjust the pH to 8.0, add 0.55 mg of a mouse anti-human IgG antibody (Shanghai Lingchao Biotechnology Co., Ltd.) that can specifically bind to the Ureaplasma urealyticum antibody, stir at room temperature for 1 hour, and add 5% bovine serum albumin by weight (BSA) closed for 1 hour, centrifuged at 12000rpm, 4°C for 30 minutes, discarded the supernatant, reconstituted with colloidal gold solution to 100ml, spread 40cm with 1ml solution 2 Spread the solution evenly on the glass fiber membrane and dry at 37°C for 30 minutes to make a colloidal gold pad.

[0026] Coating of nitrocellulose membrane

[0027] Dissolve the Ureaplasma urealyticum antigen (Shanghai Lingchao Biotechnology Co., Ltd.) that can ...

Embodiment 2

[0032] Preparation of colloidal gold pads

[0033] Prepare a colloidal gold solution with a diameter of 35nm by the chloroauric acid-trisodium citrate reduction method. After the preparation is completed, take 100ml of the colloidal gold solution in a beaker and use 0.1M K 2 CO 3 Adjust the pH to 8.2, add 1.2 mg of mouse anti-human IgG antibody (Shanghai Lingchao Biotechnology Co., Ltd.) that can specifically bind to the Ureaplasma urealyticum antibody, stir at room temperature for 1 hour, and add 5% bovine serum albumin by weight (BSA) closed for 1 hour, centrifuged at 12000rpm, 4°C for 30 minutes, discarded the supernatant, reconstituted with colloidal gold solution to 100ml, spread 40cm with 1ml solution 2 Spread the solution evenly on the glass fiber membrane and dry at 37°C for 30 minutes to make a colloidal gold pad.

[0034] Coating of nitrocellulose membrane

[0035] Dissolve the Ureaplasma urealyticum antigen (Shanghai Lingchao Biotechnology Co., Ltd.) that can spe...

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Abstract

The invention discloses a test strip for quickly detecting ureaplasma urealyticum antibodies in saliva. The test strip comprises a bottom plate on which a sample pad, a collaurum pad, a nitrocellulosefilm and a sample sucking pad are sequentially pasted, wherein the collaurum pad is attached with mouse anti-human IgG antibodies which are labeled by collaurum and can combine with the specificity of the ureaplasma urealyticum antibodies; and a detection line consisting of ureaplasma urealyticum antigens and a quality control line consisting of sheep anti-mouse secondary antibodies which can combine with the specificity of the mouse anti-human IgG antibodies are coated on the nitrocellulose film. The test strip adopts a collaurum labeling technique to detect the ureaplasma urealyticum antibodies in saliva so as to judge whether a testee infects ureaplasma urealyticum or not. The invention has the advantages of simple operation, high reaction speed and sensibility, strong specificity, suitability for on-site detection and self detection, economy, practicability, and the like.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a saliva rapid detection test strip for ureaplasma urealyticum antibody. Background technique [0002] Ureaplasma urealyticum (M. urealyticum) is the only species in the genus Ureaplasma, named for its need for urea for growth. The colony is tiny, only 15-25um in diameter, and must be observed under a low-power microscope, so it was formerly called T strain (tiny strain). There are rough particles on the surface of the colony, which can be transformed into a typical poached egg-like colony under suitable conditions. Cholesterol and urea are needed for growth, and urea is decomposed as its metabolic characteristic, producing ammonia nitrogen, which increases the pH of the medium, and the urine of patients often has a fishy smell. [0003] Ureaplasma urealyticum is most closely related to women's reproductive health. Ureaplasma urealyticum can cause genitourinary tra...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558G01N33/532G01N33/52
Inventor 郑隆泗李洪江
Owner 杭州艾力康医药科技有限公司
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