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Method for extracting monensin sodium by methanol extraction method

A technology of monensin sodium and monensin, which is applied in the field of biopharmaceuticals and engineering, can solve the problems of mass production of pure monensin sodium, achieve moderate price, improve working environment and improve operating environment Effect

Active Publication Date: 2012-01-25
SHANDONG JINYANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Domestic companies currently do not have mass production of pure monensin sodium, and almost all monensin premix products are on the market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Slant strain culture: pick a small amount of sand tube spores and inoculate them on the sterilized slant medium, cultivate them at 32°C for 240 hours, and then store them in a refrigerator at 1-6°C.

[0024] 2. Primary seed and secondary seed cultivation: inoculate 2 cultured slant strains in sterilized 500L primary seed medium under aseptic protection, cultivate at 35°C for 26 hours, and then sterilize by steam Transfer the aseptic tube into the sterilized 2000L secondary seed medium, and incubate at 35°C for 24 hours.

[0025] 3. Fermentation culture: transfer the secondary seed culture liquid into the sterilized 25,000L fermentation medium through a steam-sterilized aseptic pipeline, control the temperature at 35°C and pH at 6.4-6.8, and cultivate for 325 hours. During the cultivation process, sterilized glucose, soybean oil, etc. were added according to the metabolism to maintain the normal growth and metabolism of the bacteria. After the fermentation culture wa...

Embodiment 2

[0033] Dissolution: Weigh 200 kg of filter cake with a water content of 20%, and slowly add it into a dissolution tank with 1000L of methanol with a concentration of 70% under stirring. The monensin was fully released into methanol. Stop stirring and let stand for 15 minutes. Then the methanol solution was filtered into a decolorization tank to obtain 970L of methanol solution.

[0034] All the other technological processes are with embodiment 1, obtain 65.5 kilograms of finished products. The finished products are sampled and tested by the company's quality inspection department, and the product quality meets the current USP standards. After calculation, the yield from wet filter cake to finished product is 91%.

Embodiment 3

[0036]Dissolution: Weigh 200 kg of filter cake with a moisture content of 30%, slowly add it into a dissolution tank with 1400L of 90% methanol under stirring, control the temperature of methanol at 20°C, stir for 35 minutes, and let the filter cake The monensin was fully released into methanol. Stop stirring and let stand for 15 minutes. Then the methanol solution was filtered into a decolorization tank to obtain 1360L of methanol solution.

[0037] All the other technological processes are with embodiment 1, obtain 57.4 kilograms of finished products. The finished products are sampled and tested by the company's quality inspection department, and the product quality meets the current USP standards. After calculation, the yield from wet filter cake to finished product is 91%.

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Abstract

The invention provides a method for extracting monensin sodium by a methanol extraction method, belongs to the field of biopharmaceuticals and engineering, and is mainly used for producing a monensin pure substance (sodium salt mode) by a fermentation method. The method comprises the following steps: culturing strains in a sand soil tube; culturing strains on an inclined surface; preparing first order seed culture solution and second order seed culture solution; preparing fermentation liquor; making a filter cake; dissolving and releasing the filter cake; extracting the filter cake; and decoloring the filter cake. The method is characterized in that the process of extraction adopts mycelium with moisture content of between 20 and 30 percent, adds 5 to 8 times of methanol of which the concentration is between 60 and 90 percent to the mycelium according to weight ratio, adjusts the temperature at between 15 and 35 DEG C, and stirs the mixture for 30 to 40 minutes to ensure that the mixture is sufficiently dissolved; and a finished product is prepared through activated carbon decoloration, filtration, crystallization, separation, drying and package. The method has high product extraction rate, namely reaching over 90 percent, low cost, good product quality, namely the product is in accordance with the requirement of the monensin sodium pure substance of an American USP standard, and high economic benefit, and can be widely applied in the field of biopharmaceuticals and engineering.

Description

technical field [0001] The invention discloses a method for extracting monensin sodium by a methanol extraction method, which belongs to the field of biopharmaceuticals and engineering. It is mainly used in the production of pure monensin (sodium salt form) produced by fermentation. Background technique [0002] Monensin belongs to polyether ionophore antibiotics, also known as monensin and cinnamonycin. It is an acidic antibiotic with a pentacyclic polyether structure produced by Streptomyces cinnamonensis. It was discovered in 1967 that there are antibacterial Insecticide, it was approved for production and marketing in the United States as an anticoccidial agent in 1971, and was approved as a growth promoter for cattle and sheep in 1984. It has been promoted and applied in more than 40 countries and regions, and it is the fastest growing since the 1970s. A class of special antibiotics for livestock and poultry, with an annual usage of nearly 30,000 tons, has achieved sig...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/16C07D493/10C12R1/465
Inventor 魏向春王京光宋学宁王健刘焕钦司书俭李秀磊
Owner SHANDONG JINYANG PHARMA
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