Method for extracting monensin sodium by methanol extraction method
A technology of monensin sodium and monensin, which is applied in the field of biopharmaceuticals and engineering, can solve the problems of mass production of pure monensin sodium, achieve moderate price, improve working environment and improve operating environment Effect
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Embodiment 1
[0023] 1. Slant strain culture: pick a small amount of sand tube spores and inoculate them on the sterilized slant medium, cultivate them at 32°C for 240 hours, and then store them in a refrigerator at 1-6°C.
[0024] 2. Primary seed and secondary seed cultivation: inoculate 2 cultured slant strains in sterilized 500L primary seed medium under aseptic protection, cultivate at 35°C for 26 hours, and then sterilize by steam Transfer the aseptic tube into the sterilized 2000L secondary seed medium, and incubate at 35°C for 24 hours.
[0025] 3. Fermentation culture: transfer the secondary seed culture liquid into the sterilized 25,000L fermentation medium through a steam-sterilized aseptic pipeline, control the temperature at 35°C and pH at 6.4-6.8, and cultivate for 325 hours. During the cultivation process, sterilized glucose, soybean oil, etc. were added according to the metabolism to maintain the normal growth and metabolism of the bacteria. After the fermentation culture wa...
Embodiment 2
[0033] Dissolution: Weigh 200 kg of filter cake with a water content of 20%, and slowly add it into a dissolution tank with 1000L of methanol with a concentration of 70% under stirring. The monensin was fully released into methanol. Stop stirring and let stand for 15 minutes. Then the methanol solution was filtered into a decolorization tank to obtain 970L of methanol solution.
[0034] All the other technological processes are with embodiment 1, obtain 65.5 kilograms of finished products. The finished products are sampled and tested by the company's quality inspection department, and the product quality meets the current USP standards. After calculation, the yield from wet filter cake to finished product is 91%.
Embodiment 3
[0036]Dissolution: Weigh 200 kg of filter cake with a moisture content of 30%, slowly add it into a dissolution tank with 1400L of 90% methanol under stirring, control the temperature of methanol at 20°C, stir for 35 minutes, and let the filter cake The monensin was fully released into methanol. Stop stirring and let stand for 15 minutes. Then the methanol solution was filtered into a decolorization tank to obtain 1360L of methanol solution.
[0037] All the other technological processes are with embodiment 1, obtain 57.4 kilograms of finished products. The finished products are sampled and tested by the company's quality inspection department, and the product quality meets the current USP standards. After calculation, the yield from wet filter cake to finished product is 91%.
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