Special sterilizing and strengthening agent for sugarcane tissue culture seedlings and use method thereof

Abstract

The invention provides a special sterilizing and strengthening agent for sugarcane tissue culture seedlings and a use method thereof. The agent comprises carbendazim, naphthylacetic acid and filling materials based on mass ratio of 1000:1. The use method comprises the following steps: weighing a reagent according to effective ingredient percentage to prepare into 0.3% of solution, soaking the sugarcane tissue culture seedlings for 3min, and then temporarily planting the seedlings in a seedling bed. The test result shows that for the temporarily planted sugarcane tissue culture seedlings treated by the formula, survival rate of grade 1 seedlings is 77.57%; compared with the seedlings through conventional treatment, survived plants have obviously improved quality with plant height increasedby 104.56%, leaf length increased by 89.37%, leaf width increased by 53.85%, root length increased by 105.52%, and obviously increased roots. The formula and the use method are applicable to popularization and application in sugarcane scientific research institutions and seedling production enterprises, and the formula can be also applicable to new product development for reagent manufacturers.

Description

Technical field: [0001] The invention relates to a special bactericidal and strengthening agent for sugarcane tissue culture seedlings and a use method thereof. Background technique: [0002] Sugarcane is an important sugar crop in my country. The contribution rate of improved sugarcane varieties to the sucrose industry is over 60%. The rapid popularization and application of improved sugarcane varieties has become a direct driving force for the development of the sucrose industry. Sugarcane is a vegetatively propagated crop with a slow reproduction rate. However, the amount of seeds used in conventional cultivation is large, and the amount of seeds used per mu is as high as 8,000-10,000 buds. Therefore, the use of modern biotechnology to rapidly reproduce fine varieties has become an effective way to accelerate the rapid transformation and application of sugarcane varieties. In the actual application process, outdoor artificial planting management technology is very importa...

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a special bactericidal and strengthening agent for the artificial planting of sugarcane tissue culture seedlings and its use method, which can effectively solve the problem of low survival rate of primary seedlings in the popularization and application process of sugarcane tissue culture technology. The problem of poor seedling quality