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Method for production of lactosylceramide

The technology of a lactosyl acyl sphingosine and a manufacturing method is applied in the manufacture field of the lactosyl acyl sphingosine, can solve the problems of complicated refining process, cannot be obtained economically, cannot obtain the content, etc., and achieves the effect of expanding the scope of application

Inactive Publication Date: 2010-01-27
SNOW BRAND MILK PROD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The typical method used to produce lactosylceramide (lactosylceramide) in the past is to precipitate the glycolipid that forms a complex with the protein using an acid such as acetic acid or trichloroacetic acid, and add chloroform-methanol to the precipitate. Alcohol-containing organic solvents such as mixed liquids are used to separate and extract glycolipids from complexes with proteins, but this method cannot obtain a composition with a high content, cannot be refined efficiently, and cannot obtain lactosyl acyl groups economically. Problems with sphingosine (lactosylceramide)
[0007] In addition, as another method for producing lactosylceramide, a method of purifying a substance containing a neutral glycosphingolipid derived from cow's milk or the like with a proteolytic enzyme has been proposed. Complicated, there must be a problem of high manufacturing cost (Patent Document 1)
[0008] As described above, conventional methods for producing lactosylceramide take time and labor, and an economical method for producing highly concentrated lactosylceramide has not yet been developed.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Ganglioside (ganglioside) GM 3 Composition and ganglioside GM prepared according to its production method (Japanese Patent Laid-Open No. 5-279379) 3 500 mg of the composition was dissolved in 2 liters of water, 15% hydrochloric acid was added to adjust the pH to 3.0, and heat treatment was performed at 88° C. for 180 minutes. Then, the supernatant was neutralized with 10% potassium hydroxide and freeze-dried to obtain 372 mg of a white powder.

[0041] According to quantitative analysis using HPLC, the white powder contained 99% by weight or more of lactosylceramide.

Embodiment 2

[0043] Contains 230mg of ganglioside GD per 1 liter 3 15% hydrochloric acid was added to adjust the pH to 4.5, and calcium chloride was added to make it 0.03% by weight of the total amount, and heat treatment was carried out at 88° C. for 180 minutes. Then, the generated casein precipitate was completely removed by treatment with a centrifuge to obtain a supernatant.

[0044] The supernatant was neutralized with 10% potassium hydroxide and freeze-dried to obtain the highly concentrated lactosylceramide powder of the present invention.

[0045] As a result of measuring the lipid content contained in the obtained powder by the Rhese Gotlieb method, it contained 16% by weight of lipid.

[0046] According to quantitative analysis using HPLC, the composition contained 0.5% by weight of lactosylceramide.

Embodiment 3

[0048] Contains 230mg of ganglioside GD per 1 liter 3 15% hydrochloric acid was added to adjust the pH to 4.5, and calcium chloride was added to make it 0.03% by weight of the total amount, and heat treatment was carried out at 88° C. for 180 minutes. Then, the generated casein precipitate was completely removed by treatment with a centrifuge to obtain a supernatant.

[0049] The supernatant was neutralized with 10% potassium hydroxide, and treated with a microfiltration membrane (manufactured by Milipore) with a particle cut-off diameter of 0.1 μM to obtain a concentrate.

[0050] Then freeze-dry the concentrate to obtain the highly concentrated lactosylceramide powder of the present invention.

[0051] As a result of measuring the lipid content contained in the obtained powder by the Rhese Gotlieb method, it contained 60% by weight of lipid.

[0052] According to quantitative analysis using HPLC, 1% by weight of lactosylceramide was contained in the composition.

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PUM

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Abstract

Lactosylceramide can be produced by heat-treating ganglioside GD3 and / or ganglioside GM3 for at 70 to 100 DEG C and pH 3 to 5 for 30 to 180 minutes, adjusting the pH value of the reaction solution to pH 4.5 to 5.0, and concentrating the reaction solution with an ultrafilter membrane or the like. Lactosylceramide is useful as a reagent in the fields of medical science, pharmacy, biochemistry and the like, and is also useful as a material for a medicinal product, cosmetic product, a food, a beverage, a feed and the like.

Description

technical field [0001] The present invention relates to ganglioside (ganglioside) GD 3 and / or ganglioside GM 3 Process for producing lactosylceramide converted to lactosylceramide by heat treatment, also relates to the use of ganglioside (GD) in milk-derived raw materials 3 and / or ganglioside GM 3 A method for producing highly concentrated lactosylceramide converted into lactosylceramide and the obtained highly concentrated lactosylceramide. [0002] Lactosylceramide and highly concentrated lactosylceramide obtained by the production method of the present invention are useful as reagents in the fields of medicine, pharmacy, and biochemistry, and are also used as pharmaceuticals, cosmetics, and diets. Useful for materials such as food and feed. Background technique [0003] It is known that lactosylceramide (lactosylceramide) is a glycosphingolipid having a reducing terminal of lactose on ceramide, which exists in animal tissues, especially in nervous system organs, and i...

Claims

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Application Information

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IPC IPC(8): C07H15/04
CPCC07H15/04C07H1/00
Inventor 矶贝朋之三浦晋加藤健田中礼央村上元威吉冈俊满
Owner SNOW BRAND MILK PROD CO LTD
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