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Manganese ion diagnostic/assay kit and method for assaying concentration of manganese ions

A technology of manganese ions and kits, applied in the field of medical/food/environmental inspection and determination

Inactive Publication Date: 2010-01-13
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Neutron excitation analysis (NAA) is the best method, but can only be determined in some well-conditioned laboratories

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The manganese ion diagnosis / measurement reagent of the present embodiment is a single reagent, comprising:

[0044] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0045] Stabilizer 500mmol / L

[0046] Coenzyme 3mmol / L

[0047] Oxalate oxidase 10000U / L

[0048] Pyruvate dehydrogenase 12000U / L

[0049] Pyruvate dehydrogenase 12000U / L

[0050] Oxalic acid 15mmol / L

[0051] Acetic acid 12mmol / L

[0052] Ferrocytochrome b1 5mmol / L

[0053] Coenzyme A 3mmol / L

[0054] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0055] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the manganese ion sample to the reagent to be tested is 1 / 25, The reaction direction is positive rea...

Embodiment 2

[0058] The manganese ion diagnosis / measurement reagent of the present embodiment is a double reagent, comprising:

[0059] Reagent 1

[0060] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0061] Stabilizer 50mmol / L

[0062] Coenzyme 3mmol / L

[0063] Oxalic acid 15mmol / L

[0064] Acetic acid 12mmol / L

[0065] Ferrocytochrome b1 5mmol / L

[0066] Coenzyme A 3mmol / L

[0067] Reagent 2

[0068] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0069] Stabilizer 50mmol / L

[0070] Oxalate oxidase 10000U / L

[0071] Pyruvate dehydrogenase 12000U / L

[0072] Pyruvate dehydrogenase 12000U / L

[0073] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0074] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of ...

Embodiment 3

[0077] The manganese ion diagnosis / measurement reagent of the present embodiment is three reagents, comprises:

[0078] Reagent 1

[0079] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0080] Stabilizer 50mmol / L

[0081] Coenzyme 3mmol / L

[0082] Oxalic acid 15mmol / L

[0083] Acetic acid 12mmol / L

[0084] Ferrocytochrome b1 5mmol / L

[0085] Coenzyme A 3mmol / L

[0086] Reagent 2

[0087] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0088] Stabilizer 500mmol / L

[0089] Pyruvate dehydrogenase 12000U / L

[0090] Pyruvate dehydrogenase 12000U / L

[0091] Reagent 3

[0092] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0093] Stabilizer 500mmol / L

[0094] Oxalate oxidase 10000U / L

[0095] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0096] When measuring manganese ion concentration, set on the automatic bi...

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Abstract

The invention relates to a manganese ion diagnostic / assay kit by utilizing indirect enzymatic recycling method, enzymatic-colorimetric method and enzyme coupling method technology, also relates to a method and principle for assaying concentration of manganese ions, and compositions of a reagent, and belongs to the technical field of medical / food / environmental test assay. The kit mainly comprises the following compositions: buffer solution, coenzyme, oxalic acid, acetic acid, ferrocytochrome b1, coenzyme A, oxalate oxidase, two different pyruvate dehydrogenases and a stabilizing agent; and a sample is mixed with the reagent in certain volume ratio to generate a series of enzymatic reactions, and a reactant is put under an ultraviolet / visual analyzer for detecting the rising speed of the absorbency on a 340nm position of the main wavelength, so that the concentration of the manganese ions is measured and calculated.

Description

technical field [0001] The invention relates to a manganese ion diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring manganese ion concentration, which belongs to the technical field of medicine / food / environmental inspection and determination. Background technique [0002] As a trace element, manganese is an indispensable and necessary component in the human body, and it is also a substance that is toxic to the human body. In the natural state, it exists in divalent, trivalent and tetravalent forms, and the lower the degree of oxidation, the higher the toxicity. There are generally eight different oxidation states of manganese. The biological significance of manganese is its diverse functions as a cofactor in the human body. However, in many enzyme activation reactions, Mn 2+ and Mg 2+ Can replace each other. [0003] Commonly used graphite furnace atomic absorption spectrophotometry, atomic emission ICP-OES. Neutron exc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/25G01N21/75G01N33/84C12Q1/26
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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