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Polypeptide with function of promoting activity of fibroblast growth factor receptor 3 and screening method and application thereof

A growth factor receptor and fibroblast technology, applied in the field of peptides, can solve problems such as no FGFs, and achieve the effects of controllable quality, small molecular weight and low immunogenicity

Inactive Publication Date: 2010-01-06
INST OF FIELD OPERATION SURGERY NO 3 MILITARY MEDICL UNIV PLA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although 23 FGFs have been discovered so far, no FGFs that only specifically bind to FGFR3 have been found.

Method used

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  • Polypeptide with function of promoting activity of fibroblast growth factor receptor 3 and screening method and application thereof
  • Polypeptide with function of promoting activity of fibroblast growth factor receptor 3 and screening method and application thereof
  • Polypeptide with function of promoting activity of fibroblast growth factor receptor 3 and screening method and application thereof

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Embodiment Construction

[0028] In the present invention, phage display technology is used to screen polypeptides that can promote FGFR3 activity. Phage display technology is to insert the DNA sequence of the foreign protein or polypeptide into the appropriate position of the phage coat protein structural gene, so that the foreign gene is expressed with the expression of the coat protein. At the same time, the foreign protein or polypeptide is displayed with the reassembly of the phage. Biotechnology to the surface of phage. The foreign protein or polypeptide expressed on the surface of the phage can specifically bind to the target molecule. After the target molecule is coated on a solid-phase carrier, the phage display peptide library is screened, and the phage expressing the corresponding foreign protein or polypeptide can be separated and purified from numerous phage, and the amino acid sequence of the foreign protein or polypeptide can be Obtained by sequencing the phage DNA.

[0029] The screening...

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Abstract

The invention discloses a polypeptide with function of promoting the activity of a fibroblast growth factor receptor 3 (FGFR3) and a screening method and an application thereof. The polypeptide is formed by an amino acid sequence shown as Val-Sel-Pro-Pro-Leu-Thr-Leu-Gly-Gln-Leu-Leu-Ser. The screening method comprises the following steps: incubating a bacteriophage 12 peptide library and an elisa plate coated by the FGFR3 together, washing non-specificity combined bacteriophage, then eluting specificity combined bacteriophage and amplifying to be used as input bacteriophage elutriated in the next turn, repeating the steps for performing elutriations in three turns, extracting DNA singly cloned by the bacteriophage elutriated in the third turn and sequencing, deducting the amino acid sequence of a foreign polypeptide shown on the bacteriophage, synthesizing the polypeptide, detecting the promotion of the polypeptide to the activity of the FGFR3, and obtaining the polypeptide with function of promoting the activity of the FGFR3. The polypeptide can be used for preparing medicine of resisting low FGFR3 function low diseases.

Description

Technical field [0001] The present invention relates to a polypeptide, in particular to a polypeptide having the activity of promoting fibroblast growth factor receptor 3, as well as a screening method and application of the polypeptide. Background technique [0002] Fibroblast growth factor receptors (FGFRs) are a class of transmembrane tyrosine kinase receptors with autophosphorylation activity that bind to their corresponding ligands, fibroblast growth factors (FGFs) It plays an important role in the development of tissues and organs and in the process of damage repair. [0003] Four FGFRs have been found, namely FGFR1, FGFR2, FGFR3 and FGFR4. Among them, FGFR3 mediates the negative regulator of intrachondral ossification and plays an important role in bone development. Previous studies have shown that FGFR3 gain-of-function gene mutations inhibit the proliferation and differentiation of chondrocytes in the epiphyseal growth plate, causing a variety of genetic diseases with bo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C07K1/22G01N33/566A61K38/10A61P19/02A61P19/08A61P19/10
Inventor 陈林余瑛黄炜杜晓兰金旻戚华兵苏楠赵玲谢杨丽杨京何启芬
Owner INST OF FIELD OPERATION SURGERY NO 3 MILITARY MEDICL UNIV PLA
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