Construction method of portunus trituberculatus miers disease-resistant variety

Abstract

The invention relates to the aquatic product heredity and breeding field, in particular to a construction method of a portunus trituberculatus miers disease-resistant variety. The method comprises the following concrete steps: information of mitochondrial control zone sequence of breeding materials serves as a molecular marker, group with rich heredity mutation serves as base group of group breeding according to the molecular marker, and groups which are genetically independent serve as the supplementary materials of family breeding; first, group breeding is carried out on the base group to obtain group, family breeding is carried out on the obtained group and the supplementary materials of family breeding, then, interfamily hybridization combination is carried out on groups with stable disease-resistant character obtained from family breeding, and the family with stable disease-resistant character is the disease-resistant variety; wherein breeding materials in each step are individuals which survive with half lethal concentration after being soaked and infected by source of infection. The invention shortens the breeding time while making the most use of species genetic resources simultaneously, combines the adaptability of local crabs, and effectively avoids the inbreeding depression of small groups, thus facilitating scientifically cultivation of the portunus trituberculatus miers disease-resistant variety suitable for local aquaculture.

Description

technical field [0001] The invention relates to the field of aquatic genetics and breeding, in particular to a method for constructing a disease-resistant strain of Portunus trituberculatus. Background technique [0002] The genetic breeding of aquatic animals is traditionally carried out by group selection or crossbreeding. At present, there are also attempts to learn from the method of family selection used in poultry storage. Due to the large amount of eggs laid by general aquatic animals and the large number of offspring, family selection and breeding are very cumbersome and difficult to operate. Group selection takes a long time and has slow results. In recent years, scholars at home and abroad have been paying great attention to molecular marker-assisted breeding, but in practical applications, especially in the genetic breeding of aquatic animals, crabs have not yet achieved results. [0003] The present invention successfully develops a breeding method suitable for...

AI Technical Summary

Problems solved by technology

Due to the large amount of eggs laid by general aquatic animals and the large number of offspring, family selection is very cumbersome and difficult to operate.

Group breeding takes a long time and yields slow results

In recent years, scholars at home and abroad have been paying great attention to molecular marker-assisted breeding, but in practical applications, especially in the genetic breeding of aquatic animals, crabs have not yet achieved results

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