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Multielement high flux genetic marking system and genetic analyzing method of Chinese mitten crabs

A genetic analysis method, the technology of Chinese mitten crab, applied in the direction of biochemical equipment and methods, DNA/RNA fragments, microbial determination/inspection, etc., can solve problems that have not been systematically developed and actually applied, and achieve scientific guarantee Sexuality and sustainability, reduction of cumbersomeness, avoidance of inbreeding depression effects

Active Publication Date: 2009-12-30
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

Although a large amount of sequence information of Eriocheir sinensis has been accumulated (more than 7,000 are published in the international shared database GenBank), this information has not been systematically developed and applied to the pedigree identification and genetic relationship analysis required for genetic breeding. middle

Method used

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  • Multielement high flux genetic marking system and genetic analyzing method of Chinese mitten crabs
  • Multielement high flux genetic marking system and genetic analyzing method of Chinese mitten crabs
  • Multielement high flux genetic marking system and genetic analyzing method of Chinese mitten crabs

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Embodiment

[0044] 1) The multivariate high-throughput genetic marker system of Eriocheir sinensis is the mitochondrial sequence information system and the microsatellite molecular marker system, in which the microsatellite molecular markers are the system 1 marked by 7 EST-SSRs and the system 1 marked by 6 EST-SSRs 2 composition (see figure 1 );

[0045]System 1 is composed of 7 EST-SSR markers, and the 7 pairs of primers for amplifying the 7 EST-SSR markers are NE5 (F: CGTGAATGCTGTAGTGTAAT; R: CACCAGAAATAATGAGGTTT);

[0046] NE12(F: ATGACATTGATGCCTGACG; R: TGCCTTTATTGACCGAGAC);

[0047] NE16 (F: TTTCACATCCTCCACAGACA; R: GCCACAAGTACACCCAATCA);

[0048] NE27 (F: ACGGGAAATGGAACAGAT; R: TCCTTCCATCCTGAGTCC);

[0049] NE46 (F: ACTGCTGTTGTTGGTGGTC; R: CCTTAATCCGTTGCGTCA);

[0050] NE48(F: AAAGGTCAGTTAATTTCTTGAT; R: TCTAGTGAAATGACATATTGGT);

[0051] NE49 (F: AGACCGCTGTTATGCTCCT; R: ATGGGAATGAAGGTTATGTATG);

[0052] System 2 is composed of 6 EST-SSR markers, and the 6 pairs of primers for ...

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Abstract

The invention relates to the field of genetic thremmatology of aquatic products, in particular to a multielement high flux genetic marking system and a genetic analyzing method of Chinese mitten crabs. The genetic marking system is a mitochondria sequence information system and a microsatellite molecule marking system, wherein the microsatellite molecule marking system comprises 7 EST-SSR systems marked 1and 6 EST-SSR marked systems 2. The method comprises the following steps: later generations produced by the same parent and different parents in a polyculture family are separated according to hereditary constitution and genetic diversity through information of sequences of three pairs of primer amplifications COI, Cytb and CR of mitochondria, and male parent discrimination is performed on later generations of the discriminated parents according to genetic distance and genetic diversity through the EST-SSR microsatellite molecule marking system, thereby completing the genealogy authentication during the genetic breeding of the Chinese mitten crabs and determining the inbreeding coefficients. The invention discriminates the genetic relationship among filial generations by using the multielement high flux genetic marking system on the basis of effectively utilizing species genetic information, and effectively avoids the inbreeding recession.

Description

technical field [0001] The invention relates to the field of aquatic genetics and breeding, in particular to a multivariate high-throughput genetic marker system and a genetic analysis method for Chinese mitten crabs. Background technique [0002] With the development of biotechnology, molecular markers are constantly being updated. Currently, the markers widely used in marine animal genetics analysis are mitochondrial sequences and microsatellite markers. Mitochondrial sequence (mtDNA), as extranuclear genetic material, has the characteristics of fast evolution, non-recombination variation, and maternal inheritance. The identical sequence information indicates that the samples come from the same maternal parent. The mtDNA fragments commonly used for intraspecies genetic analysis are Cytb, COI and CR. Microsatellites (SSRs) are tandem repeat sequences composed of 2-6 bases that exist in eukaryotic and prokaryotic genomes, and are highly polymorphic and co-dominant. Microsa...

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 崔朝霞王鸿霞吴丹华刘媛李倩倩
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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