High-growth-rate Dunaliella tertiolecta obtained through ethylmethane sulfonate mutation breeding

Abstract

The invention relates to a method adopting ethylmethane sulfonate (EMS) to perform chemomorphosis on Dunaliella tertiolecta, which performs screening by using the growth rate of microalgae as an index under a normal culture condition to finally obtain a mutagenic strain ENN0001-7 with higher growth rate compared with a wild strain. Compared with the wild strain, the chlorophyll content (OD680) ofthe mutagenic strain obtained by the method is increased by 8.9 percent, and the biomass (OD750) is increased by 5.9 percent. The strain with stable and high growth rate obtained by the method provides a valuable germ plasm resource for mass production.

Description

Dunaliella with high growth rate obtained by mutagenesis with ethyl methanesulfonate technical field The present invention relates to the field of microbial engineering, in particular, the present invention relates to a method for using ethyl methanesulfonate to induce and breed Dunaliella, and a Dunaliella mutagenic strain ENN0001 with a high growth rate obtained by said method -7. Background technique Dunaliella is a kind of unicellular eukaryotic green algae without cellulose outer wall. They are mostly found in salt lakes and oceans, although they have been reported to have been found in fresh water. Dunaliella resembles Chlamydomonas, and domestic scholars classify them into the genus Euchlorophyceae, Volvox, Salinaceae, and Dunaliella. Dunaliella cells are very tiny. Judging from the dozen species of Dunaliella that have been reported so far. The largest cell is D. salina, with a volume of about 2600 μm3 (24~25×12~16 μm), and the smallest is D. minuta, which is o...

AI Technical Summary

Problems solved by technology

However, there are few breeding studies on its growth rate.

At present, the mutation breeding of Dunaliella mainly uses ultraviolet mutagenesis, but ultraviolet mutagenesis has the disadvantages of time-consuming and labor-intensive, and the probability of obtaining mutant strains is small.

Ethyl methanesulfonate (EMS), as a strong chemical mutagen, is mainly used in the mutagenesis breeding of Spirulina in algae breeding, but it has not been reported for the growth rate screening of fast-growing algal strains

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