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Preparation method for liquid composite alpha-amylase

An amylase and liquid technology, which is applied in the production, preparation and application of biological enzymes, can solve the problems of not being able to effectively improve the efficiency of catalytic degradation of starch, and the activation effect is small, so that the process parameters are easy to control, the price is reduced, and the application effect is excellent. Effect

Inactive Publication Date: 2009-11-04
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above results are often concluded under some specific conditions. Through experiments, it was found that the above-mentioned single activator has little activation effect on microbial α-amylase, and cannot effectively improve its effectiveness in catalytically degrading starch.

Method used

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  • Preparation method for liquid composite alpha-amylase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Preparation of medium temperature liquid compound α-amylase

[0021] Add 100L of medium temperature α-amylase with 10000 vigor to 500L reactor 1, and then add 150L of purified water to dilute into 4000 vigor α-amylase liquid, stir well and set aside.

[0022] Add 180L of purified water to the 500L reactor 2, then add 40 kg of sodium nitrate with a purity of 99%, stir and dissolve, then add 30 kg of polyethylene glycol (molecular weight 400), and stir for 30 minutes to obtain a mixed solution.

[0023] The α-amylase solution diluted in step 1 is added to the mixed solution in step 2, and stirred for 60 minutes to obtain a medium-temperature liquid composite α-amylase with high catalytic degradation activity.

Embodiment 2

[0025] Preparation of high-temperature liquid compound α-amylase

[0026] Add 100L of high temperature α-amylase with activity of 12000 to 500L reactor 1, and then add 100L of purified water to dilute into a liquid of α-amylase with 6000 activity, and stir well before use.

[0027] Add 240L of purified water to the 500L reactor 2, then add 30 kg of sodium nitrate with a purity of 99%, stir and dissolve, then add 30 kg of polyethylene glycol (molecular weight 400), and stir for 30 minutes to obtain a mixed solution.

[0028] The α-amylase solution diluted in step 1 is added to the mixed solution in step 2, and stirred for 60 minutes to obtain a high temperature liquid composite α-amylase with high catalytic degradation activity.

Embodiment 3

[0030] Preparation of wide-temperature liquid compound α-amylase

[0031] Add 50L of high-temperature alpha-amylase with activity of 12000 to 500L reactor 1, then add 50L of medium-temperature liquid amylase with activity of 10,000, and then add 100L of purified water to dilute to 5500-activity of alpha-amylase liquid, stir well and set aside.

[0032] Add 180L of purified water to the 500L reactor 2, then add 30 kg of sodium nitrate with a purity of 99%, stir to dissolve, then add 40 kg of polyethylene glycol (molecular weight 400), and stir for 30 minutes to obtain a mixed solution.

[0033] Add the α-amylase solution diluted in step 1 to the mixed solution in step 2, and stir and bond for 60 minutes to obtain a wide temperature liquid composite α-amylase with high catalytic degradation activity.

[0034] The test of the effect of the present invention is as follows:

[0035] 1. Determination of starch degradation efficiency by amylase

[0036] (1) Reagent

[0037] (1) Preparati...

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Abstract

The invention provides a preparation method for liquid composite alpha-amylase. Through the combination of alpha-amylase and multiple chemicals with special structures, special linking action is generated between the chemicals and the macromolecules of amylase protein so as to enhance structural stability and adhesion to substrate, thereby realizing the large promotion of the catalytic activity of the amylase. The method has the advantages of simple preparation technology, convenience in operation, no need of special equipment, little investment, little contamination, easy control of technological parameters; compared with traditional commercial amylase, the decomposition efficiency of the prepared product for starch can increase by 100% to 200%, and the cost can be greatly lowered at the same time.

Description

Technical field [0001] The present invention relates to the production, preparation and application field of biological enzymes, in particular to a method for preparing liquid composite alpha-amylase. Background technique [0002] Amylase, also known as starch degrading enzyme, can not only hydrolyze starch molecules, but also degrade starch hydrolysates such as dextrin and oligosaccharides. Alpha-amylase is widely distributed in nature, and it is contained in animals, plants and microorganisms. The first generation product BF7658 has decades of industrial production and application history. In recent years, the second generation of 70℃ medium temperature enzymes and high temperature resistant enzymes above 90℃ have been gradually developed, which are mainly prepared by the method of microbial fermentation. Many enzymes only show catalytic activity or strengthen their catalytic activity when a certain appropriate activator is present, which is called activation of enzymes. Most a...

Claims

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Application Information

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IPC IPC(8): C12N9/96
Inventor 姚继明贾佳
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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