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Method for performing variety identification and seed purity check of celery cabbage by using SCAR marker

A technology for species identification and purity inspection, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., and can solve the problems of insufficient polymorphism, long detection period and few bands.

Inactive Publication Date: 2009-10-28
NORTHWEST A & F UNIV
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  • Application Information

AI Technical Summary

Problems solved by technology

Although the results of field morphological identification are reliable and the implementation is simple, the detection cycle is long, time-consuming and labor-intensive, and it is difficult to meet the requirements of harvesting and seeding in the current year
Isoenzyme and protein electrophoresis techniques have been applied in the purity test of some cruciferous vegetable crop varieties and hybrids. Insufficient polymorphism, fewer bands, limited differences and other defects, it is difficult to meet the detection requirements of more and more varieties

Method used

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  • Method for performing variety identification and seed purity check of celery cabbage by using SCAR marker
  • Method for performing variety identification and seed purity check of celery cabbage by using SCAR marker
  • Method for performing variety identification and seed purity check of celery cabbage by using SCAR marker

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Embodiment Construction

[0037] The concrete implementation of the present invention carries out according to the following steps:

[0038] 1. Preparation of materials:

[0039] The new orange Chinese cabbage variety "Jinguan No. 2" and its parent seeds were transplanted to the field after germination and seedlings were raised. The individual plants were numbered at the seedling stage, and 100-200 plants were randomly selected, and the young leaves were taken at the seedling stage to extract DNA for later use. At the adult plant stage, the biological characters were identified according to the characteristics of the variety.

[0040] 2. DNA extraction

[0041]Using the CTAB method, the process is as follows: take 0.2g of young true leaves, grind them into powder with liquid nitrogen; add 750 μL CTAB extract and transfer to a 1.5mL centrifuge tube; Equal volume (Tris-saturated phenol: chloroform: isoamyl alcohol = 25: 24: 1) and equal volume (chloroform: isoamyl alcohol = 24: 1) were extracted once a...

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Abstract

The invention discloses a method for performing variety identification and seed purity check of a celery cabbage by using an SCAR marker. The method uses a genome DNA of a new species 'gold crown No.2' and a parent thereof of an orange celery cabbage as a template, establishes an RAPD specific finger print of a crossbreed 'gold crown No.2' crossbreed and the parent thereof through an RAPD analysis, successfully obtains a female parent specificity SCAR marker and a crossbreed specificity SCAR marker, and can perform the variety identification and the seed purity check of the 'gold crown No.2' crossbreed according to a variety finger print and specificity markers by using the two SCAR markers. The identification results of the identifications of 134 random groups of the 'gold crown No.2' accord with those of field identifications.

Description

technical field [0001] The invention belongs to the field of agricultural vegetable variety identification and improved varieties, and relates to a method for variety identification and seed purity inspection of Chinese cabbage, in particular to a method for variety identification and seed purity inspection of "Jinguan No. 2" Chinese cabbage using SCAR markers. Background technique [0002] The inspection of the genetic purity of hybrid seeds has increasingly attracted special attention from seed production units. Although the results of field morphological identification are reliable and the implementation is simple, the detection cycle is long, time-consuming and labor-intensive, and it is difficult to meet the requirements of harvesting and seeding in the same year. Isoenzyme and protein electrophoresis techniques have been applied in the purity test of some cruciferous vegetable crop varieties and hybrids. The lack of polymorphism, fewer bands, and limited differences m...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 张鲁刚王绮张明科惠麦侠张战风
Owner NORTHWEST A & F UNIV
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