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Seedling method of ulvales green algae asexual propagation

A technology of asexual reproduction and Ulva, which is applied in the field of producing Ulva green algae asexual propagation line seedlings, which can solve the problems of long seedling production cycle, unstable genetic traits, and seasonal restrictions, and achieve low production cost and short production cycle , not subject to seasonal restrictions

Inactive Publication Date: 2012-01-25
NATIONAL MARINE ENVIRONMENTAL MONITORING CENTRE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the problems referred to above, the purpose of the present invention is to establish a method for the production of clonal seedlings, which can be used for the large-scale production of Ulva order green algae, and solve the problem of long seedling production cycle, limited by seasons, unstable genetic traits, and excessive production costs. advanced questions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: Taking the Enteromorpha marginatus as material, the present invention is described in further detail.

[0017] 1. Collect wild Enteromorpha fringosa algae from the sea area, transport them back to the laboratory at low temperature in an incubator, select healthy and complete individuals, check under a microscope that there is no reproductive organ formation, wash the surface of the algae with a soft brush, and remove the attached miscellaneous algae or animal. Rinse well with sterile sea water.

[0018] 2. Select a certain area in the upper part of the algae body, cut it off, cut it with a scalpel blade or grind it with a tissue grinder, to 1-2mm (diameter), put the cut piece in a petri dish or an Erlenmeyer flask and let it stand Culture, the culture medium is PES medium, the culture condition is light intensity 55-95μmol m -2 the s -1 , temperature 20-25°C. Change the culture medium every 3 days, about a week or so, the cells on the fragments undergo...

Embodiment 2

[0019] Embodiment 2: Taking Ulva pore as material, the present invention is described in further detail.

[0020] 1. Collect vigorously growing and dark green Ulva algae bodies from natural sea areas, transport them back to the laboratory at low temperature in an incubator, select a certain size (such as 2×2cm2) area in the middle and upper part of the algae body, cut it off, and microscopically examine it. For reproductive organs, use a soft brush to scrub the surface of the algae to remove miscellaneous algae, and rinse it with sterile sea water repeatedly.

[0021] 2. Cut the cleaned algae fragments into fragments below 5mm with a blade, and culture them in a petri dish or Erlenmeyer flask. The culture medium is PES medium, and the culture conditions are light intensity of 55-95 μmol m -2 the s -1 , temperature 20-22°C. Change the culture medium every 3 days. After about 6 days, most of the vegetative cells are transformed into motile cells and released, and a small amoun...

Embodiment 3

[0022] Embodiment 3: Using pocket reef film as material, the present invention is further described in detail.

[0023] 1. Collect marsupial reef membranous algae in the vigorous growth period from natural sea areas, transport them back to the laboratory at low temperature in an incubator, and select a certain size (such as 1×1cm) in the upper part of the algae. 2 ) area, cut it off, microscopically check for no reproductive organs, use a soft brush to scrub the surface of the algae, remove miscellaneous algae, and rinse it with sterile seawater repeatedly.

[0024] 2. Use a tissue grinder to crush the cleaned algae fragments to less than 1 mm, and culture them statically in a petri dish or Erlenmeyer flask. The culture medium is PES medium, and the culture conditions are light intensity 55-95 μmol m -2 the s -1 , temperature 21-23°C. Change the culture medium every 3 days, about a week or so, except for a small part of the vegetative cells that transform into immobile cells...

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Abstract

The invention discloses a seedling method of ulvales green algae asexual propagation, comprising the following steps: wild algae body is divided into segments below threshold value (often less than 1-5mm); the segments are tissue cultured; after segment cells grow larvae with rhizoid again, the rhizoid and the larvae are stripped from fixed matrix and cultured in a aerating way after being grinded; and the tissues of the rhizoid and the larvae are induced to have facultative parthenogenesis so as to release swarm cells or fixed cells and germinate to form a new plant. Therefore, the method reaches the augmentation of clone and is used for producing seeds. The invention has outstanding advantages of short seedling production period, no limit by seasons, stable hereditary character and low production cost.

Description

technical field [0001] The invention relates to a method for mass producing green algae seedlings of Ulva order green algae through seaweed tissue culture. Background technique [0002] Ulva algae include economic species such as marsupial reef, Enteromorpha, and Ulva. The algae are rich in protein, various amino acids, vitamins, dietary fiber, and trace elements. They have the functions of lowering blood pressure, anti-tumor, and treating constipation. And other effects, welcomed by Japan and some countries in Southeast Asia. The algae of the order Ulva have a simple structure, one or two layers of cells, and the shape is thallus or tubular. The algae of this order have strong adaptability, wide temperature and wide salt, and are especially suitable for growth in eutrophic sea areas such as estuaries. Therefore, the cultivation of seaweed of Ulva order can not only bring considerable economic benefits, but also have good ecological benefits. [0003] At present, no econom...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 邵魁双巩宁闫启仑
Owner NATIONAL MARINE ENVIRONMENTAL MONITORING CENTRE
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