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Application of sasanqua extrac in weeding

An extract, Camellia oleifera technology, applied in the directions of application, herbicides and algaecides, botanical equipment and methods, etc., to achieve significant social and economic benefits, high growth inhibition effect

Inactive Publication Date: 2009-09-30
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] But there is no literature report about whether camellia oleifera has herbicidal activity

Method used

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  • Application of sasanqua extrac in weeding
  • Application of sasanqua extrac in weeding
  • Application of sasanqua extrac in weeding

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: the preparation of camellia oleifera methanol extract

[0019] Weigh 20g of the dry powder of camellia oleifera plant material into a 500mL conical flask with a stopper, add 200mL of 90% methanol, extract at room temperature for 60h, and then extract with ultrasonic vibration for 1h. Continuously extract 4 times according to the above method, combine the 4 extracts, and concentrate the extract under reduced pressure below 50°C.

[0020] Weigh 20g of camellia oleifera plant material dry powder into a 500mL conical flask with a stopper, add 200mL of 90% methanol, extract at room temperature for 72h, and then extract by ultrasonic vibration for 0.5h. Continuously extract 2 times according to the above method, combine the 2 extracts, and concentrate the extract under reduced pressure below 50°C.

[0021] Weigh 20g of dry powder of camellia oleifera plant material into a 500mL conical flask with a stopper, add 200mL of 90% methanol, extract at room temperature...

Embodiment 2

[0021] Weigh 20g of dry powder of camellia oleifera plant material into a 500mL conical flask with a stopper, add 200mL of 90% methanol, extract at room temperature for 71h, and then extract by ultrasonic vibration for 45 minutes. Continuously extract 2 times according to the above method, combine the 2 extracts, and concentrate the extract under reduced pressure below 50°C. Example 2 Camellia oleifera methanol extract to the indoor activity assay (bioassay method) of weeds

[0022] Use 90% methanol to disperse and dissolve any of the extracts prepared in Example 1 to different volumes, and prepare active test solutions with different concentrations to be tested. For example, when the solution is fixed to 80 mL, the active test solution to be tested is prepared with a concentration equivalent to 0.25 g dry sample / mL solvent.

[0023] Take a petri dish with a diameter of 12 cm, put two layers of Xinhua brand qualitative filter paper with a diameter of 12 cm on it, select 20 so...

Embodiment 3

[0037] Embodiment 3: The herbicidal activity test of the methanol extract of camellia oleifera to the grass plants

[0038] The present embodiment takes potted barnyardgrass as an example.

[0039] 1. Bioassay method

[0040] Pre-emergence (soil spray) treatment method: put mud in a small plastic basin with a diameter of 22 cm, and after settling for 3 days, pour off the water layer. Three concentrations were set for treatment: 0.02, 0.01, and 0.005 g / mL methanol crude extract, and three replicates were set for each treatment. Put 25 barnyardgrass seeds in each pot, spray 10mL of medicine into each pot with a portable sprayer, and spray 10mL of water as a control. After 8 days and 16 days of treatment, the number of barnyardgrass germination was counted, the stem length was measured, and the inhibition rate was obtained.

[0041] Post-emergence (stem and leaf spray) treatment method: put mud in a small plastic basin with a diameter of 22 cm, settle for 3 days, and pour off ...

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Abstract

The invention discloses an application of sasanqua extract in weeding, which can be applied to the preparation of a growth inhibitor or weedicide of barnyard grass, oat grass, redroot pigweed, descurainia Sophia, and the like, can obviously inhibit the biological activity of various grasses and has remarkable weeding effect. According to the invention, efficient green agriculture weedicide without pollution can be developed, and remarkable social benefit and economic benefit can be obtained.

Description

technical field [0001] The invention belongs to the technical field of plant growth inhibitors or herbicides, and specifically relates to a new application of camellia oleifera extract in inhibiting grass growth or weeding. Background technique [0002] Plants are a natural treasure house of biologically active compounds. Humans have a long history of using plants to prevent and control harmful organisms, and have accumulated rich experience. From ancient times, plants were directly used in practical production to modern day to find active compounds and artificially transform them into products. Pesticides, human research and discussion on botanical pesticides has gone through a long process. But so far, there are relatively few botanical herbicide products. With further technological and social development, the research on botanical herbicides has greater potential. [0003] Camellia oleifera (Camellia oleifera Abel) is a unique woody tree species in my country. It is dist...

Claims

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Application Information

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IPC IPC(8): A01N65/08A01P13/00
Inventor 钟国华胡美英郝卫宁李琳陈少华
Owner SOUTH CHINA AGRI UNIV
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