Alcohol extract of walnut kernel and use thereof

Abstract

The invention relates to an alcohol extract of walnut kernel and a use thereof, effectively developing a new use of the alcohol extract of walnut kernel capable of inhibiting propagation of nerve tumour cells and applicable to the preparation of anti-tumour medicines to control propagation of tumour cells. The inventive alcohol extract of walnut kernel is prepared by the following steps: soaking the walnut kernel in absolute ethyl alcohol for one weak, the weight of which is 4-10 times of that of the absolute ethyl alcohol; filtering the solution to obtain medical liquid; condensing the medical liquid at 55-65 DEG C; drying the medical liquid in a vacuum environment at 55-65 DEG C to obtain 7.34% of brown oil-form alcohol extract of walnut kernel; while storing, dissolving the obtained extract by dimethyl sulfoxide into storage liquid, the concentration of which is 300 mug / mul; and storing the storage liquid in a refrigerator at -20 DEG C for standby use. In experiment, the storage liquid is prepared into needed concentration by serum-free culture medium that satisfies demand of cultured cells; and the storage liquid is filtered to remove bacteria therein and is added with serum. Experiment shows that the inventive alcohol extract of walnut kernel has function of inhibiting propagation of multiple cancer cell strains and is expected to be a new product for research on anti-cancer medicine development.

Description

1. Technical field [0001] The invention relates to the field of medicine, in particular to a walnut kernel alcohol extract and its application. 2. Background technology [0002] Cancer is the primary disease that threatens human health. The uncontrolled proliferation of cancer cells is the reason for its intractability. Growth factors combine with the corresponding receptors on the cell membrane to transmit growth signals to the nucleus to cause normal cell growth. Abnormally enhanced cell growth signals are the cause of cancer. The key reason for the uncontrolled proliferation of cells. Inhibiting the signal transmission of cancer cell growth is an important way for the development of anticancer drugs. Finding drugs to control the proliferation of cancer cells has always been a difficult problem in medical research. [0003] The inventor used the water maze experiment to detect the memory of animals to observe the impact of walnut kernels on the memory of young mice, old mi...

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Problems solved by technology

[0004] In view of the above situation, the object of the present invention is to provide a kind of walnut kernel alcohol extract and its application, effectively open up the new application of walnut kernel alcohol extract, can inhibit neurotumor cell proliferation, can be used in the preparation of anticancer drugs, solve the problem of The problem of controlling the proliferation of cancer cells, walnut kernel alcohol extract is, add walnut kernels to 4-10 times the weight of walnut kernels in absolute ethanol and soak for a week, then filter to get the medicinal solution, concentrate the medicinal solution at 55-65°C, 55-65 ℃ vacuum drying to obtain brown oily walnut kernel ethanol extract with a yield of 7.34%; during storage, dissolve it into a stock solution with a concentration of 300 μg / μl with dimethyl sulfoxide (DMSO), and store it in a -20 ℃ refrigerator for future use

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