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Abstraction method for ben oil

A kind of Moringa oil, Moringa technology, applied in the field of Moringa oil extraction, water enzymatic extraction of Moringa oil, can solve problems such as protein denaturation, complex equipment, many steps, etc., achieve low acid value and peroxide value, Ease of handling, low phospholipid content

Inactive Publication Date: 2009-08-19
THE RES INST OF RESOURCES INSECTS RIRI OF THE CHINESE ACADEMY OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this process can extract more than 95% of the oil in the oil, it has many steps and complicated equipment. What is more important is that after the oil is extracted, the protein in it is severely denatured and difficult to use.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A, the Moringa oleifera seeds are pulverized, and sieved with a 60-mesh standard inspection sieve to obtain the Moringa oleifera seed kernel powder;

[0024] B, take by weighing A step gained Moringa kernel powder 50g, press Moringa kernel powder: the mass ratio of distilled water=1: 5, add distilled water 250g in Moringa kernel powder, stir 2min with the speed of 4000r / min, with a concentration of 0.1mol / L hydrochloric acid adjusts the pH value of the mixture to 4;

[0025] C, in the mixture of step B, add cellulase 0.05g according to the mass ratio of Moringa oleifera kernel powder: enzyme = 1: 0.001, 30 ℃ of constant temperature enzymolysis reactions in water bath 8h, keep stirring simultaneously, stirring speed 40r / min;

[0026] D, the mixture that is enzymatically hydrolyzed through step C is placed in a centrifuge and centrifuged under the condition of a rotating speed of 2000r / min for 2min to separate three layers, wherein the upper layer is a free oil layer with...

Embodiment 2

[0030] A, the Moringa oleifera seeds are pulverized, sieved with a 60-mesh standard inspection sieve, to obtain the Moringa oleifera seed kernel powder;

[0031] B, take by weighing A step gained Moringa kernel powder 500g, press Moringa kernel powder: the mass ratio of distilled water=1: 7, add distilled water 3500g in Moringa kernel powder, stir 5min under the speed condition of 6000r / min, use Concentration is 0.1mol / L hydrochloric acid adjustment mixture pH value is 5.0;

[0032] C, in the mixture of step B, add pectinase 5g according to the mass ratio of Moringa kernel powder: enzyme = 1: 0.01, 50 ℃ of constant temperature enzymolysis reactions in water bath 12h, keep stirring simultaneously, stirring speed 100r / min;

[0033] D. Place the mixture that has been enzymatically hydrolyzed in step C in a centrifuge for centrifugation. Under the condition of a rotating speed of 3000r / min, centrifuge for 5 minutes and separate into three layers, wherein the upper layer is a free ...

Embodiment 3

[0037] A, the Moringa oleifera seeds are pulverized, sieved with a 60-mesh standard inspection sieve, to obtain the Moringa oleifera seed kernel powder;

[0038] B, take by weighing A step gained Moringa kernel powder 2kg, press Moringa kernel powder: the mass ratio of distilled water=1: 12, add distilled water 24kg in Moringa kernel powder, stir 8min under the rotating speed condition of 8000r / min, use The concentration is 0.1mol / L NaOH to adjust the pH value of the mixture to 8;

[0039] C, in the mixture of step B, add alkaline protease 100g by the mass ratio of moringa kernel powder: enzyme=1:0.05, 60 ℃ of constant temperature enzymolysis reaction 48h in the water bath, constantly stir simultaneously, stirring speed 140r / min;

[0040] D. Put the enzymatically hydrolyzed mixture in step C into a centrifuge for centrifugation. Under the condition of centrifuge speed of 4000r / min, centrifuge for 8 minutes and separate into three layers, wherein the upper layer is a free oil l...

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Abstract

The invention provides a method for extracting horseradish tree oil from horseradish tree seeds, which is characterized by comprising the following steps: horseradish tree kernel powder sieved with 60 meshes of a standard test sieve is blended with water according to the mass ratio of horseradish tree kernel powder: water equaling to 1:5-12, the Ph value of the mixture is adjusted to be 4-8, the mixture is added with enzyme according to the mass ratio of the horseradish tree kernel powder: enzyme equaling to 1:0.001-0.05, and then reacts for 8-48 hours in an enzymolysis way in water bath of 30-60 DEG C normal temperature; the mixture is separated and dissociation oil layer at the upper layer is separated; emulsion is centrifugalized again; the secondary dissociation oil layers are combined to obtain the horseradish tree oil. The horseradish tree oil which can be directly eat is obtained in the condition that an organic solvent is not used, has high purity, low phosphatide content, light color, and low acid number and over oxidation value. The method for extracting horseradish tree oil is safe, efficient, energy-saving and environment-friendly.

Description

technical field [0001] The invention relates to a method for extracting moringa oil, in particular to a method for extracting moringa oil from moringa seed kernels with an enzymatic method, and belongs to the field of biotechnology. Background technique [0002] Moringa seeds are rich in oil, and the extracted Moringa oil is brighter in color and is a yellow transparent liquid containing 76% monounsaturated fatty acids, mainly oleic acid, which is resistant to repeated frying and is a good cooking oil. In addition, the viscosity of Moringa oil is lower than other commonly used oils, and has the lowest viscosity, which is very suitable for use as a fragrance excipient, not only as a high-quality raw material for cosmetics, spices, and preservatives, but also widely used in industrial production Among them, such as: in industrial applications, Moringa oil can be used as a good lubricant for precision machinery, and can also be used as a mechanical lubricant under special cond...

Claims

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Application Information

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IPC IPC(8): C11B1/10
Inventor 段琼芬马李一张重权王有琼
Owner THE RES INST OF RESOURCES INSECTS RIRI OF THE CHINESE ACADEMY OF FORESTRY
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