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Cell-activating agent, collagen production-promoting agent, bleaching agent, anti-oxidizing agent, anti-inflammatory agent, aromatase activity-promoting agent, protease activity-promoting agent, skin

A technology of cell activator and skin external application, which can be used in skin care preparations, cosmetics, food preparation and other directions, and can solve problems such as undiscovered

Active Publication Date: 2009-07-22
NOEVIR CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Furthermore, no information has been found on cell stimulators, collagen production accelerators, whitening agents, antioxidants, anti-inflammation agents, and aromatase activity accelerators that use Hibiscus makinoi extract as an active ingredient. , skin external preparations and prior art of food

Method used

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Examples

Experimental program
Comparison scheme
Effect test

manufacture example 1

[0047] A 20-fold amount of 50% by mass ethanol was added to the dried ground product of bark or leaves of Hibiscus makishima, extracted with stirring at room temperature for 2 hours, and insoluble matter was removed by filtration. After concentration under reduced pressure, freeze-drying was carried out to obtain the extract of Hibiscus makishima.

manufacture example 2

[0049] Add 20 times the amount of purified water to the dry crushed bark, leaves, or fruit of Hibiscus makishima, heat at 120° C. for 20 minutes with an autoclave, and extract. After removing insoluble matter by suction filtration while maintaining high temperature, freeze-drying was performed to obtain an extract of Hibiscus makishima.

[0050] Using the extract of hibiscus makishima obtained in the above-mentioned production example, experiments for evaluating each action were performed.

Embodiment 1

[0051] [Example 1] Evaluation experiment of cell activation effect on dermal fibroblasts

[0052] In this evaluation experiment, the bark extract of hibiscus makishima obtained by the production method described in production example 1 was used as a sample. Evaluation was performed in the following order. Sow normal human dermal fibroblasts on 96-well microplate, 2.0×10 per well 4 indivual. As the seeding medium, a product obtained by adding 1% by mass of fetal bovine serum (FBS) to Dulbeck's modified Eagle's medium (DMEM) was used. After culturing for 24 hours, the medium was replaced with sample culture solutions prepared with various sample concentrations in DMEM medium supplemented with 1% by mass of FBS, and cultured for an additional 48 hours.

[0053] Then, replace with the culture medium containing 400 μg / mL 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) for 2 hours, and use 2 - Propanol extraction of formazan resulting from the opening of the t...

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PUM

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Abstract

Disclosed is a cell activator, a collagen production promoter, a skin whitening agent, an antioxidant agent, an antiinflammatory agent, an aromatase activity promoter, a protease activity promoter, an external preparation for the skin, or a food which comprises an extract of Hibiscus makinoi.

Description

technical field [0001] The present invention relates to a cell activating agent, a collagen production accelerator, a whitening agent, an antioxidant, an anti-inflammation agent, an aromatase activity accelerator, a protease activity accelerator, and external use for skin containing an extract of Hibiscus makinoi medicines and food. Background technique [0002] Causes of aging symptoms such as decreased skin elasticity and wrinkles and blemishes due to aging include decreased cell function, decrease or degeneration of extracellular matrix components such as collagen, and melanin production due to ultraviolet rays Or pigmentation and oxidative damage to cells. In order to prevent or improve such aging symptoms, various active ingredients have been searched for and combined with research. It is well known that there is the essence of tangerine as a cell stimulator (see Patent Document 1), as a collagen production accelerator, there is an extract extracted from the buds of a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/97A61Q1/12A61Q5/06A61Q5/12A61Q19/00A61Q19/02A61Q19/08A61Q19/10
CPCA61K2800/522A23V2002/00A61K8/97A61Q19/02A61Q19/08A23L1/3002A23L33/105A61K8/9789A23V2250/21
Inventor 菊池宏子荒岛雅树荒卷沙也香吉田浩子
Owner NOEVIR CO LTD
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