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Rebaudioside a composition and method for purifying rebaudioside a

A technology of steviolbioside and composition, applied in the field of steviolbioside A composition and its purification

Active Publication Date: 2009-07-01
THE COCA-COLA CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these prior art methods fail to provide substantially pure steviolbioside A compositions using a single recrystallization step

Method used

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  • Rebaudioside a composition and method for purifying rebaudioside a

Examples

Experimental program
Comparison scheme
Effect test

Embodiment A1

[0080] The crude steviol glycoside A (77.4% purity) mixture was obtained from a commercial source. Impurities (6.2% steviol glycosides, 5.6% steviol diglycoside C, 0.6% steviol diglycoside F, 1.0% other steviol glycosides, 3.0% steviol diglycoside D, 4.9% steviol diglycoside B, 0.3% steviol diglycoside) In the dry state (water content of 4.7%), it was confirmed and quantified by HPLC.

[0081] Combine crude stevioside A (400g), ethanol (95%, 1200ml), methanol (99%, 400ml) and water (320ml) and heat to 50°C for 10 minutes. The clear solution was allowed to cool to 22°C for 16 hours. The white crystals were filtered and washed twice with ethanol (2×200 ml, 95%), and dried in a vacuum oven at low pressure (20 mm) at 50° C. for 16 to 24 hours.

[0082] The final composition (130 g) of the substantially pure steviol diglycoside A contains 98.91% steviol diglycoside A, 0.06% steviol diglycoside, 0.03% steviol diglycoside C, 0.12% steviol diglycoside F, and 0.13% other steviol glycosides ...

Embodiment A2

[0084] Crude stevioside A (80.37% purity) was obtained from commercial sources. Impurities (6.22% steviol glycosides, 2.28% steviol glycosides C, 0.35% steviol glycosides A, 0.78% steviol glycosides F, 0.72% other steviol glycosides, 3.33% steviol glycosides B, 0.07% steviol glycosides) In the dry state (moisture content 3.4%), it was confirmed and quantified by HPLC.

[0085] Combine crude stevioside A (100g), ethanol (95%, 320ml), methanol (99%, 120ml) and water (50ml) and heat to 30-40°C for 10 minutes. The clear solution was allowed to cool to 22°C for 16 hours. The white crystals were filtered and washed twice with ethanol (2×50 ml, 95%). The moist filter cake (88 g) was slurried in ethanol (95%, 1320 ml) for 16 hours, filtered, washed with ethanol (95%, 2×100 ml), and placed in a vacuum oven at low pressure (20 mm) 60 Dry at ℃ for 16 to 24 hours.

[0086] The final composition (72 g) of the substantially pure steviol diglycoside A contains 98.29% steviol diglycoside A, 0.03%...

Embodiment A3

[0088] Crude stevioside A (80.37% purity) was obtained from commercial sources. Impurities (6.22% steviol glycosides, 2.28% steviol glycosides C, 0.35% steviol glycosides A, 0.78% steviol glycosides F, 0.72% other steviol glycosides, 3.33% steviol glycosides B, 0.07% steviol glycosides) In the dry state (moisture content 3.4%), it was confirmed and quantified by HPLC.

[0089] Combine crude stevioside A (50g), ethanol (95%, 160ml), methanol (99%, 60ml) and water (25ml) and heat to about 30°C for 10 minutes. The clear solution was allowed to cool to 22°C for 16 hours. The white crystals were filtered and washed twice with ethanol (2×25 mL, 95%). The moist filter cake (40 g) was slurried in methanol (99%, 600 ml) for 16 hours, filtered, washed with methanol (99%, 2×25 ml), and placed in a vacuum oven at low pressure (20 mm) 60 Dry at ℃ for 16 to 24 hours.

[0090] The final composition (27.3 g) of the substantially pure steviol diglycoside A contains 98.22% steviol diglycoside A, 0....

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PUM

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Abstract

Exemplary embodiments of this invention encompass a method for purifying crude rebaudioside A. In particular, this invention relates to a method for purifying crude rebaudioside A compositions comprising purities from approximately 40 % to approximately 95 % rebaudioside A to obtain a substantially pure rebaudioside A product with a single crystallization step. Resulting polymorph and amorphous forms of rebaudioside A and methods for preparing polymorph and amorphous forms of rebaudioside A from crude rebaudioside A compositions and substantially pure rebaudioside A compositions also are disclosed.

Description

Technical field [0001] The present invention generally relates to polymorphic and amorphous stevioside A, methods for purifying stevioside A, and methods for preparing polymorphic and amorphous stevioside A. More specifically, the present invention relates to polymorphic and amorphous steviol glycosides A, and methods for purifying or crystallizing steviol glycosides A with aqueous organic solvents or organic solvents to obtain high-yield and high-purity products. Background technique [0002] Steviol diglycoside A is a high-sweetness diterpene glycoside sweetener, which has the following chemical structure: [0003] [0004] Stevia diglycoside A and other steviol glycosides are isolated and extracted from the stevia plant (Stevia rebaudiana (Bertoni)) (Stevia). These plants are cultivated in Japan, Singapore, Taiwan, Malaysia, South Korea, China, Israel, Cash crops in India, Brazil, Australia and Paraguay. Steviol diglycoside A is an alternative non-calorie sweetener, its func...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/236C07H1/08A23L27/30
Inventor 英德拉·普拉卡什格兰特·E·杜波依斯乔治·A·金马尼·厄普雷提
Owner THE COCA-COLA CO
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