Siphoning type intermittent spraying bioreactor

Abstract

The invention relates to a siphon type batch spray bioreactor belonging to the biotechnology field, wherein a port of a liquid storage bottle is connected to a gas-liquid mixture agitation tank by a first liquid pump, a gas nozzle is arranged at internal bottom of the gas-liquid mixture agitation tank, a gas flow meter outside the gas-liquid mixture agitation tank is connected to the gas nozzle, an output end of the gas-liquid mixture agitation tank is connected to an input end of a spray device, an output end of the spray device and an input end of the reactor main body, an output end of the reactor main body is connected to a continuous harvesting apparatus, a waste liquid output port of the reactor main body is connected to a waste liquid bottle, the continuous harvesting apparatus is connected to a reclaiming end of the reactor main body through a second liquid pump, an oxygen probe and a pH probe are arranged in the reactor main body and the oxygen probe and the pH probe are connected to a computer. The invention has advantages of simple design, convenient operation and low cost.

Description

technical field [0001] The invention relates to a device in the field of biotechnology, in particular to a siphon type intermittent spray bioreactor. Background technique [0002] my country is one of the countries with the richest medicinal plant resources in the world. As the earliest natural resources developed, medicinal plant resources are currently facing two crises: one is that the reserves and output of wild plant resources are generally declining; the other is that The demand for medicinal plants continues to increase, resulting in excessive mining of medicinal plant resources. On the one hand, the traditional field cultivation of medicinal plants is affected by "time and place", resulting in large fluctuations in yield and quality; on the other hand, it inevitably brings about problems such as pesticide residue pollution. The use of bioreactors for plant tissue and cell in vitro culture has the characteristics of rapid growth and easy control, which can gradually r...

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Problems solved by technology

[0004] Found through the retrieval of prior art literature, " Controlling Hyperhydration of Carnations (Dianthus caryophyllus L.) Grown in a Mist Reactor " (in spray reaction) published by M.J.Correll etc. on " Biotechnology and bioengineering " (biotechnology and bioengineering) Controlling the hydration of carnations in the device), a spray reactor for cultivating organisms is proposed in this paper. The spray device is designed on the basis of acoustics, and the spraying work is controlled by the acoustic device. The spray device needs to be equipped with sophisticated intelligent control Instruments are expensive and complicated to operate

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