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Construction of chimera PRRSV, compositions and vaccine preparations

A composition and replicon technology, applied in the field of molecular virology, can solve problems such as no cross protection

Inactive Publication Date: 2009-06-10
PROTATEK INT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, conventional vaccines do not cross-protect against other forms of genetically varied (diversified) PRRSV isolates

Method used

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  • Construction of chimera PRRSV, compositions and vaccine preparations
  • Construction of chimera PRRSV, compositions and vaccine preparations
  • Construction of chimera PRRSV, compositions and vaccine preparations

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1. Addition of genetic marker sequences in infectious non-virulent PRRSV clones.

Embodiment 2

[0035] Example 2. Subcloning of the 5' sequence (13117 bp) of an avirulent strain of PRRSV in pBluescript SK(+).

Embodiment 3

[0036] Example 3. Subcloning of the 3' sequence of a wild isolate of PRRSV in the vector pCR-Blunt.

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PUM

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Abstract

Chimeric replicons of North American Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) containing the 5' sequence of an avirulent strain of PRRSV and a 3' sequence of a virulent strain of PRRSV are provided. Further provided is a method of producing attenuated PRRSVfrom the chimeric replicon. Also provided are compositions containing the replicon or attenuated virus. Vaccines and a method of vaccinating pigs against PRRSV are also provided.

Description

technical field [0001] The present invention relates to molecular virology. In particular, the invention includes methods of attenuating the virulence of porcine reproductive and respiratory syndrome virus (PRRSV) and viral compositions comprising attenuated PRRSV. Background technique [0002] Porcine Reproductive and Respiratory Syndrome (PRRS) is the most significant disease economically affecting the swine industry, with the virus causing approximately $5.6 billion in annual losses in the United States. Primary symptoms of the disease are reproductive problems in sows and gilts, including late abortions, stillbirths and mummies, as well as small, frail piglets that are born viremic and often do not survive. In addition, symptoms in young pigs manifest as respiratory disease resulting in fever, lethargy, dyspnea, low appetite, slow growth and occasional death, often in combination with other respiratory pathogens. Semen from infected boars, whether through natural matin...

Claims

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Application Information

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IPC IPC(8): C12N7/04A61K39/12
CPCC12N2770/10022C12N7/00A61K2039/525A61K2039/5258C07K14/005C12N2770/10021
Inventor X·张S·马
Owner PROTATEK INT
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