Immunodetection kit for detecting porcine reproductive and respiratory syndrome virus and application thereof

Abstract

The invention provides an immunodetection kit for detecting porcine reproductive and respiratory syndrome virus (PRRSV) and an application thereof. The kit comprises 96-well PRRSV N protein-coated ELISA (enzyme-linked immunosorbent assay) plate, a washing solution, rabbit anti-pig enzyme-linked antibodies, a color-development solution, a stop solution, positive control, and negative control. The immunodetection kit adopts a genetic engineering method to construct virus-specific neutralizing epitope polypeptide fragment, and adopts an ELISA technology. The kit can effective distinguish various antibodies, constructs the technical standards for evaluating immunoprotection effect, is very important for the prevention and control of porcine reproductive and respiratory syndrome, and has broad application market. The immunodetection kit is convenient in operation, has low use cost and good repeatability, and is suitable for large-scale generalization and application.

Description

technical field [0001] The invention relates to a porcine reproductive and respiratory syndrome immune detection kit and application thereof. Background technique [0002] Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the main infectious diseases of pigs. It is characterized by abortion, stillbirth and mummification of sows in late pregnancy, death of newborn piglets, and substantial A disease characterized by pneumonia. PRRSV replicates mainly in mononuclear macrophages and the lymphatic system, disrupting the function of these cells and ultimately destroying the immunity of infected pigs. The result will lead to the decline of resistance to other viruses and the mixed infection of multiple pathogenic microorganisms such as Pasteurella multocida, Streptococcus suis, Haemophilus parasuis and Salmonella, resulting in the death of a large number of pigs. Since the second half of 2006, the "high fever" caused by PRRSV mutants has broken out and continu...

AI Technical Summary

Problems solved by technology

As a result, the resistance to other viruses will decrease and a variety of pathogenic microorganisms such as Pasteurella multocida, Streptococcus suis, Haemophilus parasuis and Salmonella will cause mixed infections, resulting in the death of a large number of pigs

Since the second half of 2006, the "high fever" caused by PRRSV mutants has broken out and continued to spread in my country. Except for a very few pig farms with good isolation conditions and high management levels, almost 100% of the pig farms have been affected. It is estimated that there are More than 40 million pig farms died in this outbreak, while some medium and small pig farms were forced to close, causing huge economic losses to the pig industry

Existing detection methods can only detect all antibodies induced by PRRSV in animals, but cannot distinguish their epitope specificity. Therefore, although high antibody titers are sometimes detected, the level of neutralizing antibodies is very low, which still plays a role. no protection

[0007] (2) The relationship between neutralizing antibodies and immunity needs to be further clarified

[0012] (3) Existing inspection methods cannot meet the needs of prevention and control

[0014] Existing detection reagents have certain defects. They cannot distinguish between antibodies produced by wild virus infection and antibodies produced by vaccine immunization, nor can they distinguish whether antibodies produced after vaccine immunization are protective antibodies or non-protective antibodies. Therefore, the detection data cannot really judge It is impossible to make a correct assessment of the immune effect of the vaccine whether the pig herd has protection against PRRSV, which is the most important reason restricting the widespread use of existing detection reagents