Immunity detection reagent kit for rice black-streaked dwarf fijivirus in single-head small brown rice planthopper

Abstract

The invention discloses an immunodetection kit specially used for rice black-streaked dwarf virus in single planthopper and a using method thereof. Specific polyclonal antibodies of the rice black-streaked dwarf virus (RBSDV) are utilized to build a dot immuno-binding assay (DIBA) detecting method which is used for building the detection kit for rice black-streaked dwarf virus, main reagents of the kit are working solution or concentrated solution, and the kit has the characteristics of convenient use, simple operation, and the like. And simultaneously, the use of the rice black-streaked dwarf virus detection kit can be a standard program or a reduced program, which can greatly broaden the using ranges and applicable persons of the kit and facilitate the population and application of the technique to establishment units, and further, the kit is applied in the fields of plant disease prognosis and prediction and the formulation of prevention and curing strategies, and the like. In addition, the kit can be used for measuring the inoculation strength in varietal resistance seed selection of rice black-streaked dwarf virus, and the like.

Description

technical field [0001] The invention relates to an immunological detection kit specially used for rice black-streaked dwarf virus in single-headed brown planthopper and its application in agriculture, which belongs to the field of agricultural science and technology. Background technique [0002] Rice black-streaked dwarf virus (Rice black-streaked dwarf virus, RBSDV) is a viral disease transmitted by the striatellus striatellus. It infects rice and causes rice black-streaked dwarf disease. Once the rice is infected, it will not be harvested. Since the disease was discovered in Yuyao County, Zhejiang Province, my country in 1963, it has mainly occurred in indica rice-producing areas in southern Zhejiang in the last century, but it has rarely been found in major japonica rice-producing areas such as Jiangsu. At the beginning of 2004, rice stripe blight broke out in Jiangsu and other provinces, and a batch of disease-resistant varieties such as Zhendao 88, Xudao 3 and Yandao 8...

AI Technical Summary

Problems solved by technology

At present, the methods used in the world to detect the virus-carrying rate of SBPH include ELISA, RT-PCR, Northern hybridization, etc., but RT-PCR and Northern hybridization are high in cost and are not suitable for large-scale detection of samples; The RBSDV detection method is fast, sensitive, simple and easy to implement. It is expected to detect a RBSDV detection technology suitable for the application of grassroots agricultural technicians in my country. It can carry out early detection and timely warning of poisonous insects in the early stage. It can also be applied to RBSDV, Research on the basic field of interaction between SBPH and plant host

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