Gene order relating to cordycepin biological synthesis
A technology of biosynthesis and cordycepin, applied in plant gene improvement, genetic engineering, biochemical equipment and methods, etc., can solve the problems of unsatisfactory incremental effect and long cycle, and achieve the effect of increasing the content and promoting the expression of synthetase
Active Publication Date: 2009-05-06
JONJEE HI TECH INDAL & COMML HLDG
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Problems solved by technology
Its shortcoming is that the conventional traditional method mentioned above has a long cycle and unsatisfactory incremental effect
Method used
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[0096] specific implementation plan
[0097] The preparation method of the gene related to the biosynthesis of cordycepin according to the present invention
[0098] (1) Screening of mutant strains
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Abstract
The invention relates to cordycepin biosynthetic related genes and application thereof. By adopting a suppression subtractive hybridization (SSH) technique, seven cordycepin biosynthetic related genes which are named as chcs1, chcs2, chcs3, chcs4, chcs5, chcs6 and chcs7 respectively are cloned, wherein the length of cDNA sequence of the chcs2 is 774bp. Through constructing an expression transformative vector of a high-efficiency plant containing the genes, means of biosynthetic gene copy amount in thalli is increased, and the content of cordycepin thalli for expressing cordycepin is improved, so as to meet requirement on medical development.
Description
[0001] This application is a divisional application with the filing date of September 06, 2005, the application number of 200510037011.8, and the title of "gene sequence related to the biosynthesis of cordycepin". technical field [0002] The invention relates to a secondary metabolite biosynthesis gene of the Cordyceps fungus in the field of bioengineering, in particular to a gene related to the biosynthesis of cordycepin. Background technique [0003] Cordyceps fungi are an important class of entomogenous fungi. Cordycepin is one of the important active ingredients of Cordyceps sinensis and Cordyceps militaris. It has unique effects in antibacterial, antiviral, antitumor, insecticidal and other aspects. Development of valuable nucleoside antibiotics. More than 100 documents have been published abroad to introduce the research on anti-tumor, anti-virus, anti-bacterial and extraction and purification of cordycepin. [0004] In 1951, Americans such as Cuningham (Cuningham) ...
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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/31C12N15/80C12P19/40
Inventor 叶小舟吴继明莫红丽邹爱国
Owner JONJEE HI TECH INDAL & COMML HLDG
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