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High-efficiency method for fermentation production of L-glutamic acid

A technology of glutamic acid and fermentation liquid, which is applied in the field of high-efficiency fermentation to produce L-glutamic acid, can solve the problems of low sugar-acid conversion rate and low fermentation acid production, and achieve significant economic benefits, increase production, and easy operation Effect

Inactive Publication Date: 2009-04-08
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the shortcomings of the existing glutamic acid production method, such as low fermentation acid production and low sugar-acid conversion rate, and provide a method for efficient fermentation of L-glutamic acid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The microbial strains used are existing glutamic acid producing bacteria. High-efficiency fermentation is carried out with the above-mentioned strains, and there are two types of media used:

[0018] (1) Seed medium: 2.5% glucose, 3% corn steep liquor, 2% bean concentration, K 2 HPO 4 ·3H 2 O 0.2%, MgSO 4 ·7H 2 O 0.1%, urea 0.025%. Adjust the pH to 7.0-7.2 with NaOH and hydrochloric acid, and sterilize at 115°C for 15 minutes.

[0019] (2) Fermentation medium: glucose 8%, sugar cane molasses 0.1%, corn steep liquor 0.4%, MgSO 4 ·7H 2 O0.15%, Na 2 HPO 4 12H 2 O 0.2%, KCl 0.1%, MnSO 4 0.0001%, FeSO 4 0.0001%, V B1 0.00001%. Adjust the pH to 7.0-7.2 with NaOH and hydrochloric acid, and sterilize at 115°C for 15 minutes.

[0020] Insert the strains into the seed medium with an inoculum size of 5%; at 32°C, pH 7.0 and dissolved oxygen 20%, culture in a 5L automatic control fermenter for 6 hours to the logarithmic phase, and inoculate at 10% The amount is i...

Embodiment 2

[0022] The microbial strains used are existing glutamic acid producing bacteria. High-efficiency fermentation is carried out with the above-mentioned strains, and there are two types of media used:

[0023] (1) Seed culture medium: with embodiment 1.

[0024] (2) Fermentation medium: 10% glucose, 0.15% sugarcane molasses, 0.6% corn steep liquor, MgSO 4 ·7H 2 O 0.18%, Na 2 HPO 4 12H 2 O 0.25%, KCl 0.13%, MnSO 4 0.0002%, FeSO 4 0.0002%, V B1 0.00002%. Adjust the pH to 7.0-7.2 with NaOH and hydrochloric acid, and sterilize at 115°C for 15 minutes.

[0025]Insert the strains into the seed medium with an inoculum size of 5%; at 32°C, pH 7.0 and dissolved oxygen 20%, culture in a 5L automatic control fermenter for 6 hours to the logarithmic phase, and inoculate at 15% The amount is inserted into a 5L automatic control fermenter containing fermentation medium, and the temperature of the fermentation broth is controlled by a sequential temperature raising mode: 34°C for 0...

Embodiment 3

[0027] The microbial strains used are existing glutamic acid producing bacteria. High-efficiency fermentation is carried out with the above-mentioned strains, and there are two types of media used:

[0028] (1) Seed culture medium: with embodiment 1.

[0029] (2) Fermentation medium: glucose 12%, sugarcane molasses 0.2%, corn steep liquor 0.8%, MgSO 4 ·7H 2 O 0.2%, Na 2 HPO 4 12H 2 O 0.3%, KCl 0.15%, MnSO 4 0.0003%, FeSO 4 0.0003%, V B1 0.00003%. Adjust the pH to 7.0-7.2 with NaOH and hydrochloric acid, and sterilize at 115°C for 15 minutes.

[0030] Insert the strains into the seed medium with an inoculum size of 5%; at 32°C, pH 7.0 and dissolved oxygen 20%, culture in a 5L automatic control fermenter for 6 hours to the logarithmic phase, and inoculate at 20% The amount is inserted into a 5L automatic control fermenter containing fermentation medium, and the temperature of the fermentation broth is controlled by a sequential temperature raising mode: 34°C for 0-5...

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Abstract

The invention relates to a method for high-efficiency fermentation production of L-glutamic acid. The method can solve the problems of lower acid production, low glucose-acid conversion rate, and the like of the prior production process. The method comprises the following steps: a biotin auxotroph strain is taken as a production strain, according to a certain proportion, a plurality of nutritional components are prepared into a culture medium with rich and balanced nutrients as the fermentation culture medium, the dissolved oxygen is controlled on the appropriate level through the regulation of the stirring speed of a fermentation tank and the wind amount, the pH is controlled by feeding ammonia water, and the residual sugar is controlled on the lower level by feeding glucose solution with a certain concentration, and the fermentation is stopped till 32h. The method can shorten the whole fermentation cycle and greatly improve the yield (more than 150g / l) and the conversion rate (more than 60 percent) of the L-glutamic acid under the situation of not increasing any additional equipment and manpower investment, the whole process has simple operation and lower production cost, thereby being very applicable to industrial production.

Description

【Technical field】: [0001] The invention relates to a method for efficiently fermenting and producing L-glutamic acid, belonging to the technical field of producing amino acids by fermentation. 【Background technique】: [0002] In the entire amino acid production industry, the production of glutamic acid occupies an extremely important position, and its output accounts for more than 2 / 3 of the total amino acid output. Monosodium glutamate is monosodium glutamate, which has a strong umami taste and is currently the most used condiment in the world. In addition, glutamic acid is also widely used in medicine, cosmetics and industry. Glutamic acid fermentation is a typical metabolically controlled fermentation. After Kinoshita et al. successfully produced glutamic acid by microbial fermentation in 1957, microbial fermentation has become the main method of glutamic acid production, and its industrial scale has also expanded year by year. At present, the production technology of g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/14
Inventor 陈宁徐庆阳宋翔谢希贤刘淑云
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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