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Uses of bagasse in immobilization fibre bed reactor for fermentation production of organic acid as immobilization material

A technology of reactor and organic acid, applied in the application field of bagasse as an immobilization material, to achieve the effect of ensuring stability and good tolerance

Inactive Publication Date: 2009-04-08
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Bagasse, as the waste left after sugarcane juice extraction in the sugar industry, is rich in fibrous structure and is a natural immobilization material, but so far there is no report on its application in organic acid fermentation as an immobilization material

Method used

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  • Uses of bagasse in immobilization fibre bed reactor for fermentation production of organic acid as immobilization material
  • Uses of bagasse in immobilization fibre bed reactor for fermentation production of organic acid as immobilization material

Examples

Experimental program
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Effect test

Embodiment 1

[0046] Embodiment 1 built-in plant fiber bed reactor produces propionic acid

[0047] Propionibacterium freudeenreichii NX-4 (Propionibacterium freudeenreichii NX-4, Chinese patent publication number: CN101182457A, strain preservation number: CCTCC No.M 207015), which has been patented by our laboratory, is produced in a built-in plant fiber bed reactor propionic acid. Seed medium: glucose 20g / L, peptone 5g / L, yeast extract 5g / L, NaCl 5g / L, pH6.9. Fermentation medium: peptone 10g / L, yeast extract 5g / L, NaCl 3g / L, (NH 4 ) 2 SO 4 5g / L, KH 2 PO 4 5g / L, pH6.9.

[0048] The bagasse was immobilized in the built-in immobilization column 11a, and the seed solution of P. freundii NX-4 was inserted into the seed medium pre-loaded with a volume of 3.6L according to the inoculum size (0.4L) of 10% (v / v). In the reactor 10 (total volume: 4L), the temperature is 35°C, and 0.1L / min N 2, utilize stirring device 9 to stir (rotating speed 100r / min), promote the constant exchange of int...

Embodiment 2

[0049] Embodiment 2 built-in cotton fiber bed reactor produces propionic acid (comparative example 1)

[0050] The bacterial species, culture conditions and feeding method are the same as those in Example 1, except that the immobilized material is changed from bagasse to cotton fiber. The built-in cotton fiber bed reactor was used to produce propionic acid. After 496 hours of fermentation, the output of propionic acid was 67.0g / L, and the production efficiency was 0.14g / L / h.

Embodiment 3

[0051] Embodiment 3 built-in plant fiber bed reactor produces propionic acid

[0052] Bacterial classification, culture condition are with embodiment 1. The initial glucose concentration is 100g / L, when the glucose concentration is lower than 10g / L, add 80g / L glucose again through the constant flow pump 5-2 (total glucose concentration: 180g / L), ferment for 350h, and the output of propionic acid is 74.3g / L, the production efficiency reaches 0.21g / L / h.

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Abstract

The invention discloses an application of bagasse as an immobilized material in an immobilized fibrous bed reactor applied to fermenting and producing an organic acid. The bagasse as the immobilized material not only can be applied to an internal immobilized fibrous bed reactor but also to an external immobilized fibrous bed reactor. The bagasse applied by immobilized cells is a waste material in sugar industry, therefore the cost as the immobilized material is rather low. After the bagasse is crushed, the specific surface area is increased, cells can be adsorbed on fiber surfaces and in fiber clearance in abundance and can fall off automatically when cells are in degeneration or when cell viability is relatively poor, which guarantees the viability of cells adsorbed at fiber. The production efficiency of the organic acid of the immobilized fibrous bed reactor which takes the bagasse as the immobilized material is far higher than that of a traditional gel entrapment method as well as than other internal and external vegetable fiber bed reactors which are currently reported with the highest production rate.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and relates to the application of bagasse as an immobilization material in the production of organic acids by an immobilized fiber bed reactor. Background technique [0002] Organic acids refer to a class of compounds with a carboxyl group (-COOH) in their molecular structure, and have broad application prospects in the fields of food, feed, and plastics. Traditional organic acid production methods mainly use petroleum as raw material to produce by chemical synthesis. At present, the biological preparation of organic acids has been favored more and more due to the advantages of natural products and abundant raw materials. However, there are still many problems in organic acid fermentation production. Organic acid microbial fermentation, such as propionic acid, lactic acid, butyric acid and succinic acid fermentation production, generally has problems such as slow bacterial prod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/36C12M1/02C12P7/52C12P7/56C12P7/46
CPCC12M23/20C12M25/18C12M29/18C12M41/26C12M47/10
Inventor 徐虹冯小海陈飞李莎欧阳平凯
Owner NANJING UNIV OF TECH
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