Nitroso mercaptan fluorescent quantitative determination kit

A fluorescence quantitative detection and nitrosothiol technology, which is applied in the direction of fluorescence/phosphorescence, chemiluminescence/bioluminescence, and analysis by making materials undergo chemical reactions, so as to save the experimenter's time and simplify the complicated steps.

Inactive Publication Date: 2009-03-25
HUAZHONG NORMAL UNIV
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  • Abstract
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Problems solved by technology

At present, there is no kit available for the quantitative detection of SNO at home and abroad

Method used

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  • Nitroso mercaptan fluorescent quantitative determination kit

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Embodiment Construction

[0016] Below in conjunction with accompanying drawing, the composition of the present invention and using method are described in further detail:

[0017] The composition of the detection kit:

[0018] Reagent bottle 1: 1.5mL EP tube packed in a black plastic bag, and DAN solution dissolved in 0.62M HCl is sealed inside the tube. Accurately weigh 0.05mg DAN, fully dissolve in 1mL 0.62M HCl solution, this is reagent I.

[0019] Reagent bottle 2: A 1.5mL EP tube packed in a black plastic bag, and the inside of the tube is sealed and packed with a DAN solution of 0.62M HCl containing 3.7mM mercuric chloride. Accurately weigh 0.001g of mercuric chloride, dissolve it in 20μL DMSO (dimethyl sulfoxide), and dissolve it together with 0.05mgDAN in 1mL of 0.62M HCl solution. This is reagent II.

[0020] Reagent bottle 3: white plastic bottle, sealed with NaNO inside the bottle 2 standard solution. Accurately weigh 0.0069g NaNO 2 , dissolved in 10 mL double-distilled water to obtain...

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Abstract

The invention relates to a fluorescent quantitative detection reagent kit for s-nitrosothiol, which comprises four reagent bottles which are filled with four reagents and are a reagent bottle 1, a reagent bottle 2, a reagent bottle 3 and a reagent bottle 4 respectively, a kit body (6), a kit cover (7), retaining plates provided with holes (5), and a reagent kit specification (8), wherein the reagent bottles are separated and fixed through the retaining plates provided with holes (5); a descriptive label is arranged on each reagent bottle; and the accurately weighed reagents with fixed weight proportions are hermetically enclosed into the reagent bottles, wherein 1 millimeter of 2, 3-diaminonaphthalene solution which is dissolved into 0.62 mass of HCl is filled into the reagent bottle 1; 1 millimeter of 2, 3-diaminonaphthalene solution which is dissolved into 0.62 mass of HCl and contains mercuric chloride is filled into the reagent bottle 2; a NaNO2 standard solution is filled into the reagent bottle 3; and 2.8 masses of NaOH solution is filled into the reagent bottle 4; and the reagent bottle 1 and the reagent bottle 2 are 1.5 millimeters of EP tubes which are packaged by black plastic bags. The reagent kit is simple, convenient and flexible to detect the s-nitrosothiol level of various cell samples.

Description

technical field [0001] The invention relates to a kit for quantitative detection of nitrosothiols, which is suitable for basic research on the etiology and pathogenesis of diseases related to nitrosothiols and nitric oxide signaling pathways, and is suitable for various environmental or chemical factors. Quantitative detection of changes in nitrosothiol levels. Background technique [0002] S-nitrosothiol (SNO) is an ester of nitric oxide and thiol compounds, which plays an important role in nitric oxide signaling pathway. The formation of SNO can preserve the biological activity of NO and regulate protein activity. Under physiological and pathological conditions, the SNO signaling pathway may be up-regulated or down-regulated. Therefore, nitrosothiols are very important as biomarkers for certain diseases the value of. [0003] At home and abroad, we have been working on effective SNO detection methods for many years. Some of the existing methods have low detection sensiti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/76
Inventor 曹毅杨旭李岩丁书茂袁均林
Owner HUAZHONG NORMAL UNIV
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