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Method for detecting goat fertility

A technology for fecundity and goats, applied in the direction of biochemical equipment and methods, microbe determination/inspection, etc., can solve problems such as no discovery

Inactive Publication Date: 2009-01-07
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Description
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AI Technical Summary

Problems solved by technology

The cAMP response element (CRE) is present in the rat and human GnRHR genes but not in the mouse GnRHR gene

Method used

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  • Method for detecting goat fertility
  • Method for detecting goat fertility
  • Method for detecting goat fertility

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Experimental program
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Embodiment 1

[0030] Embodiment 1, the establishment of the method for detecting goat fecundity and its effect verification

[0031] 1. Establishment of a method for detecting goat fecundity

[0032] 1) template material preparation

[0033] The blood samples of 130 Jining green goat ewes with lambing records in 2005 were collected from the Jining green goat breeding base of the Ministry of Agriculture (Jiaxiang County, Shandong Province); the blood samples of 50 Inner Mongolia cashmere goat ewes were collected from Inner Mongolia white cashmere goats Breeding sheep farm (Etuoke Banner, Ordos City, Inner Mongolia); blood samples of 30 Boer goat ewes were collected from the Animal Breed Improvement Station of Anhui Province; blood samples of 40 Angora goat ewes were collected from Qinshui Demonstration Ranch in Jincheng City, Shanxi Province Zhengzhuang Town, Qinshui County, China). Among them, 130 Jining Qinggoat ewes randomly selected were descendants of 5 rams, and their numbers were 24...

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Abstract

The invention discloses a method for detecting the productive capacity of a goat. The method is to detect the 757 bit nucleic acid of a GnRHR gene in the gene group of the detected goat to be G or be mutated into A and confirms the genotype of the goat, then confirms the productive capacity of the goat by the genotype; if the 757 bit nucleic acid of the GnRHR gene in the gene group of the detected goat is G, the genotype of the homozygote thereof is AA; when the 757 bit nucleic acid of the GnRHR gene in the gene group of the detected goat is A, the genotype of the homozygote thereof is BB; the genotype of the heterozygous thereof is AB; the productive capacity of the goat of BB genotype is better than that of the goats with AB genotype and AA genotype. The method of the invention utilizes inheritance to mark the polymorphism for confirming the productive capacity of the goat, can fast and conveniently confirm the productive capacity of the goat of Jining and provides an accurate and simple method for detecting the productive capacity of the goat for the breeding of the goat.

Description

technical field [0001] A method for testing the fertility of goats. Background technique [0002] Gonadotropin releasing hormone (GnRH), a decapeptide, is a key neuroendocrine regulator of the hypothalamus-pituitary-gonad axis, and the function of GnRH depends on the gonadotropin releasing hormone receptor receptor, GnRHR). GnRHR is a high-affinity G protein-coupled receptor located on the surface of gonadotropic cells. GnRH and GnRHR combine on the surface of gonadotropic cells to promote the synthesis and release of follicle-stimulating hormone (FSH) and luteinizing hormone in the anterior pituitary gland. Hormone (luteinizing hormone, LH). The ovulation of mammals depends on the LH peak, and the increased secretion of GnRH and GnRHR will cause the LH peak. Therefore, the GnRH gene and the GnRHR gene play an important role in the regulation of reproduction in mammals. [0003] Yang-Feng et al. (1986) used fluorescence in situ hybridization to map the human GnRH-I gene ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 储明星狄冉方丽马月辉李奎
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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