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Method for extracting polysaccharide and flavone from Gynura divaricata

A technology of bracken and flavonoids, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of unreported extraction process, achieve good separation effect, low cost and high yield

Inactive Publication Date: 2009-01-07
SHANXI PUJING BIOLOGY TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Modern scientific research has shown that P. chinensis is rich in polysaccharides and flavonoids biologically active substances. At present, there are many studies on the determination of active ingredients in P.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for extracting polysaccharides and flavonoids from Azalea, the steps are:

[0024] (1) Take 50g of stir-fried white coriander leaves, remove impurities and pulverize them, add water and stir evenly after pulverization, leaching once at a constant temperature at a liquid-to-solid ratio of 25:1, at a temperature of 80°C, and for 4 hours, and then filter through a 300-mesh filter cloth get the extract;

[0025] (2) the extract is concentrated in vacuum with a rotary evaporator, and the volume of the concentrated solution is 1 / 4 of the volume of the extract;

[0026] (3) Adding 4 times the volume percentage concentration of the concentrated solution volume to the concentrated solution is 95% edible ethanol aqueous solution, then let it stand for 10 hours to precipitate the polysaccharide, and centrifuge the alcohol-precipitated solution at 3600r / min for 10min, Obtain precipitation and supernatant respectively;

[0027] (4) Dissolving the separated precipitate wit...

Embodiment 2

[0032] A method for extracting polysaccharides and flavonoids from Azalea, the steps are:

[0033] (1) Take 50g of naturally dried white cod leaves, crush them, add water and stir evenly, extract once at a constant temperature at a liquid-to-solid ratio of 20:1, at a temperature of 80°C, and for 6 hours, and then filter through a 300-mesh filter cloth to obtain the extract ;

[0034] (2) Concentrating the extract in a vacuum with a rotary evaporator, so that the volume of the concentrate is 1 / 4 of the volume of the extract;

[0035] (3) Add 4 times the volume percentage of the concentrated solution to the concentrated solution and add an aqueous edible ethanol solution with a volume percentage concentration of 95%, then let it stand for 10 hours to precipitate the polysaccharide, filter the alcohol precipitation solution, and obtain the precipitate and the filtrate respectively;

[0036] (4) Dissolving the separated precipitate with water to 500ml and then freeze-drying to ob...

Embodiment 3

[0041] A method for extracting polysaccharides and flavonoids from Azalea, the steps are:

[0042] (1) Take 200g of stir-fried white coriander leaves, grind them, add water and stir them evenly, extract twice at a constant temperature under the conditions of a liquid-to-solid ratio of 20:1, a temperature of 80°C, and a time of 4 hours, filter through a 300-mesh filter cloth to obtain the extract, and combine Extraction solution;

[0043] (2) Concentrate the extract in vacuum with a rotary evaporator, so that the volume of the concentrate is 1 / 3 of the volume of the extract;

[0044] (3) Add 4 times the volume percentage concentration of the concentrated solution to the concentrated solution and be 95% ethanol aqueous solution, then let it stand for 8 hours to precipitate the polysaccharide, and then centrifuge the alcohol-precipitated solution at 3600r / min for 10min , to obtain precipitation and supernatant respectively;

[0045] (4) Vacuum-dry the separated precipitate to o...

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Abstract

The invention discloses a method for extracting polysaccharides and flavones from Gynura divaricata, which comprises the following steps of: (1) collecting leaf or stem of Gynura divaricata as raw material, removing impurities, pulverizing, extracting with water at a constant temperature, and filtering to obtain an extractive solution; (2) concentrating the extractive solution to obtain a concentrated solution; (3) adding a precipitant to the concentrated solution to separate out the polysaccharides precipitate, and separating the precipitate and the supernatant; (4) drying the precipitate to obtain Gynura divaricata polysaccharides extract; (5) recovering the solvent from the supernatant, adding water to the substrate to obtain flavones crude extractive solution; (6) purifying the flavones crude extractive solution with a resin column, eluting with distilled water, eluting with ethanol aqueous solution until no flavones are detected in the eluate, and mixing the ethanol eluates; and (7) recovering ethanol under reduced pressure, and drying the substrate to obtain flavones extract. The inventive method has the advantages of rapidness, convenience, low cost, high yield, etc.

Description

technical field [0001] The invention belongs to the field of natural product extraction, and relates to a method for simultaneously extracting polysaccharides and flavonoids from radishes. Background technique [0002] Gynura divaricata (L.) DC., also known as white back soil notoginseng, fork flower soil notoginseng, perennial herbaceous plant, Compositae notoginseng, 30-50cm high, massive rhizome, purple-red stem , with short hairs, distributed in the south and southwest of my country, and found in all parts of Fujian, and likes to be born in humid shade. The whole plant eliminates heat and detoxifies, promotes blood circulation and removes stasis, removes wind and reduces swelling, and its roots are used as medicine. It has the effects of purging fire, cooling blood, and promoting body fluid. It is a common Chinese herbal medicine for treating various inflammations such as mastitis, arthritis, and tracheitis. [0003] Modern scientific researches have shown that Chinese c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P11/00A61P15/14A61P19/02C08B37/00C07D311/30C07H1/08A61K127/00A61K135/00
Inventor 刘凌孙慧吴娜崔明学周明
Owner SHANXI PUJING BIOLOGY TECH
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