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Preparation method of extracting and separating potato vine polysaccharide from natural plant sweet potato vine

A natural plant, sweet potato vine technology, applied in the field of medicine, can solve the problems of complex ingredients, low bioavailability, and low relative content of active ingredients

Inactive Publication Date: 2008-12-31
ZHEJIANG ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

We have also found through investigation that the single prescription of sweet potato vine decoction in this province is effective in treating hyperlipidemia, but there are several problems as follows: (1) The extraction process needs to be improved. Complex, low relative content of active ingredients, low bioavailability, large dosage
(2) There is no certain quality control technology
(3) The active ingredients need to be confirmed

Method used

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  • Preparation method of extracting and separating potato vine polysaccharide from natural plant sweet potato vine
  • Preparation method of extracting and separating potato vine polysaccharide from natural plant sweet potato vine
  • Preparation method of extracting and separating potato vine polysaccharide from natural plant sweet potato vine

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Experimental program
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Embodiment

[0078] Embodiment: the preparation method of yam polysaccharide FST

[0079] According to the optimum result of orthogonal test, it is determined that the best extraction process is 100kg of dried sweet potato vines, chopped and decocted twice, each time for 1 hour. Combine the decoction liquids, concentrate under reduced pressure, add 3 times the volume of 95% ethanol for precipitation, and dry to obtain the crude yam polysaccharide. Gel filtration method removes inorganic salts and small molecular impurities, ethanol or acetone solvent method removes macromolecular impurities. Then use the Sevag method to remove the protein, that is, accurately weigh an appropriate amount of the sample, add an appropriate amount of distilled water to dissolve it ultrasonically, add chloroform-n-butanol solution (4:1) to make 1 / 4ml of the aqueous solution, place it in a separatory funnel, and shake it fully for 5 Minutes later, it was centrifuged at 4000r / min for 1min in a centrifuge, and th...

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Abstract

The invention belongs to the technical field of medicine, and more particularly relates to a method for preparing sweet potato vine amylase which is used for decreasing blood fat of a patient with hyperlipidemia and is extracted and separated from natural sweet potato vine. The steps are as follows: the sweet potato vine is dried; inorganic salt and small molecule are removed by gel filtration method; macromolecule impurity is removed by ethanol or acetone solvent method; protein is removed by Sevag method; a mixture of amylase is obtained, which is the sweet potato vine amylase (FST). Three polysaccharide compounds (F1, F2, F3) are obtained after the molecular weight of crude amylase is separated in subsection by column chromatography of SePhadex. The method has the advantages that the technology is simple and convenient; the quality control specification has direction; the obtained mixture has the effect on decreasing blood fat.

Description

technical field [0001] The invention belongs to the technical field of medicine, in particular to a preparation method for extracting and separating yam vine polysaccharide from natural plant sweet potato vine, which is used for hypolipidemic effect of diseases such as hyperlipidemia. Background technique [0002] Diseases such as hyperlipidemia and diabetes have become the number one killer of human health, and their incidence rate is getting higher and higher, and the age of onset is getting lower and lower. Finding new active ingredients from natural products, especially plant ingredients, is an effective way to innovate drugs. It not only has great potential for discovering new drugs, but also provides unique chemical structures for designing more ideal new drugs. Therefore, many countries and pharmaceutical companies have gradually shifted the focus of lipid-lowering drug development to natural medicines. [0003] Sweet potato vine, also known as red call vine and fan ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/39A61P3/06C08B37/00A61K127/00A61K135/00
Inventor 张信岳李钦陈爱君陆红
Owner ZHEJIANG ACAD OF MEDICAL SCI
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