Preparation for detecting organophosphorus pesticide residual test paper and its use method

Abstract

The present invention discloses a preparation method of a test paper for testing the residual organphosphorous pesticide and a using method thereof, wherein the preparation method comprises the following procedures: (1) preparing acetylcholine esterase with fresh blood of hen or female duck as raw material; (2) fixing the acetylcholine esterase on the filter paper; (3) slicing when the filter paper is frozen and dried. A substrate liquid, a color developing liquid and a sensitization liquid are provided. The principle is that the inhibition of the organphosphorous pesticide which is processed by the sensitization liquid to the acetylcholine esterase in the chicken or duck blood serum to make that the substrate of naphthyl acetate of the acetylcholine esterase can not be decomposed thereby that the displayed colors are not consistent when the fast blue B is added into to obtain the purpose of determining whether the sample contains the residual organphosphorous pesticide. The method of the invention has the advantages of low cost, simple and fast operation. The method is suitable for determining whether the residual organphosphorous pesticide exists in vegetable, fruit and food on-site.

Description

Technical field: [0001] The invention belongs to the field of analytical chemistry and in particular relates to the preparation and application method of a test paper for detecting organophosphorus pesticide residues. Background technique: [0002] At present, pesticide residues have become a major problem threatening food safety. It is very urgent to increase the detection of pesticide residues and strengthen the monitoring of pesticide residues in foods such as vegetables and fruits on the market. Pesticide residue analysis traditionally mainly uses gas chromatography, gas chromatography-mass spectrometry, and high-performance liquid chromatography, which can measure the residues more accurately, but requires large-scale instruments, cumbersome pre-treatment operations, and specially trained personnel. Moreover, the instrument is inconvenient to carry and move, and it is difficult to adapt to the requirements of simple and rapid on-site and large-scale sample residue anal...

AI Technical Summary

Problems solved by technology

Pesticide residue analysis traditionally mainly uses gas chromatography, gas chromatography-mass spectrometry, and high-performance liquid chromatography, which can measure the residues more accurately, but requires large-scale instruments, cumbersome pre-treatment operations, and specially trained personnel. In addition, the instrument is not convenient to carry and move, and it is difficult to adapt to the requirements of simple and fast on-site and large-scale sample residue analysis