Method for detecting aqueous solution oxalate

Abstract

The invention provides a method for detecting oxalate in water solution. The method uses pyrocatechol violet as color developing reagent based on Cu<2+> ions and directly and quantificationally detects the oxalate in the water solution in HEPES buffer solution with a pH of 6.0, thereby the determining process is simple and convenient and the measuring result is accurate. The method has high sensibility, can not be interfered by other anions in the water solution, and can be applied to detection of the oxalate in clinic, foodstuff and environment.

Description

A method for detecting oxalate in aqueous solution Technical field: The invention relates to a method for detecting oxalate, in particular to a method for detecting oxalate in an aqueous solution. Background technique: Oxalic acid is a highly toxic compound that widely exists in plants, animals, and microorganisms. Its accumulation in the body can cause various pathological conditions, such as urolithiasis, hyperoxaluria, and renal failure. Increased oxalate concentration in urine may be a symptom of diabetes and other diseases, and decreased may be acute and chronic nephritis. Excessive oxalic acid in the daily diet can form insoluble matter with calcium before absorption, hindering the absorption of calcium; after absorption, it can form stones with calcium and other substances. It is reported that 70%-80% of kidney stones are mainly composed of calcium oxalate. Beverages sterilized by ozone will form oxalic acid when the content of vitamin C is high, thereby promoting ...

AI Technical Summary

Problems solved by technology

1. The titration method has low sensitivity, and the operation is cumbersome and time-consuming;

2. Other colorimetric methods require pre-precipitation, it is difficult to completely extract oxalate, and the operation is cumbersome and the repeatability is poor;

3. Capillary electrophoresis instruments are not popular yet;

4. High-performance liquid chromatography requires the separation of oxalate, and the interference of inorganic acids is serious during the separation process, and the equipment is expensive, and the requirements for operators are high;

5. The high cost of oxalate oxidase limits the wide-scale application of this method

Images