Medicine for preventing and curing liver injury

A technology for liver damage and liver damage, applied in the direction of drug combinations, pharmaceutical formulas, medical preparations containing active ingredients, etc.

Inactive Publication Date: 2008-10-15
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] But up to now, there is no report about icariin as a drug for preventing and treating liver damage

Method used

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  • Medicine for preventing and curing liver injury
  • Medicine for preventing and curing liver injury
  • Medicine for preventing and curing liver injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Preparation of icariin (see Ye Haiyong et al., Zhejiang University Journal (Medical Science), 2005, 34(2): 131-136)

[0049] Take 2 kg of Epimedium sagittarius collected in the mountains of southern China, grind it into powder, and then extract it with 95% ethanol (10 L). 250 g of concentrated material were obtained, which was then further extracted with 2.5 L of chloroform, ethyl acetate and n-butanol. Pack 8 g of ethyl acetate extract and 10 g of n-butanol extract into a silica gel chromatography column, and wash with CHCl 3 -MeOH-HCOOH (15:1:0.5) and CHCl 3 -MeOH (8:2) eluted separately. Through this process, 300 mg of icariin and noricariin precursor icariin can be obtained. The precursor is then hydrolyzed according to the method briefly described below to obtain icariin. Solution A: 80 mg precursor was ultrasonically dissolved in 18 ml methanol solution; Solution B: 500 U (0.433 g) cellulase was dissolved in 180 ml (0.1 mol / L), pH 5.0 acetate buffer. Solution...

Embodiment 2

[0051] Icaritin attenuates H in rat primary hepatocytes 2 o 2 damage model test

[0052] 1. Acquisition and culture of primary rat hepatocytes

[0053] Refer to Seglen's two-step collagenase perfusion method and make improvements (Xu Zhe et al., Journal of Postgraduate Medicine, 2003, 16(5): 342-344). SD rats (purchased from the Experimental Animal Center of the Second Military Medical University) were anesthetized and injected with 1000 U of heparin into the tail vein. The abdominal cavity was opened under aseptic conditions, and the distal end of the portal vein and inferior vena cava and the superior vena cava were separated and ligated. Intubate at the end of the heart, cut off the proximal end of the inferior vena cava, and open the perfusion with 4°C D-Hanks solution (containing 0.5mol / L EDTA) at a constant flow rate of 40ml / min. After the liver turns khaki, put the Put the liver in a sterilized plate, use 300ml of 0.03% collagenase at 37°C, and circulate perfusion at...

Embodiment 3

[0056] Icaritin attenuates CCl in rat primary hepatocytes 4 damage model test

[0057] 1. See Example 2 for the acquisition and culture of primary rat hepatocytes.

[0058] 2. Rat primary hepatocytes were cultured in 24-well plates and randomly divided into CCl 4 Injury group, solvent DMSO group, icariin and reduced glutathione control group, icariin 2 μmol / L treatment group, icariin 4 μmol / L treatment group, icariin 8 μmol / L treatment group , Icaritin 16μmol / L treatment group. Each group has 6 replicate wells. Reduced glutathione is a commonly used hepatoprotective drug in clinical practice, and it was set as the positive control group together with icariin in this experiment. Two days after primary rat hepatocytes were cultured, culture medium with different concentrations of drugs were added to each group, and the CCl-containing medium was replaced after 24 hours. 4 The culture medium (concentration is 8mmol / L), after acting for 6 hours, the cell culture supernatant wa...

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PUM

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Abstract

The invention relates to a medicine with icaritin as the active component for treating hepatic injuries. The pharmacological experiment shows that the icaritin has antiapoptotic, antioxidant and antiphlogistic activities and protective effects on hepatic injuries; and has evident inhibiting effects on the release of active oxygen, cell apoptosis factor and inflammatory cell factor caused by acute and chronic hepatic injury, anoxic hepatic injury and ischemia-reperfusion hepatic injury. The acute toxicity test of the icaritin on a mouse shows that the icaritin has no toxicity. Therefore, the icaritin has wide development prospect, and can be used for preparing new drugs for preventing and treating the acute and chronic hepatic injury, the anoxic hepatic injury and the ischemia-reperfusion hepatic injury.

Description

technical field [0001] The invention relates to a medicine for preventing and treating liver damage. Background technique [0002] Epimedii Herba (Epimedii Herba) is a traditional Chinese medicine in my country. It is a plant of the genus Epimedium in the Berberidaceae family (Berberidaceae). Flavonols in Epimedium have pharmacological activities such as treating cardiovascular and cerebrovascular diseases and preventing osteoporosis (Pei Likuan et al., Chinese Journal of Traditional Chinese Medicine, 2007, 32(6): 466-471). Icaritin (icaritin) is the aglycone of icariin, the main active ingredient in Epimedium, and belongs to flavonol compounds. It is not only the product of icariin transformed by chemical treatment, but also the An important metabolite in the body (Liu J et al, J Pharm Biomed Anal, 2004, 36(2): 365-370). [0003] As a chemical molecular monomer, icariin is currently reported on its pharmacological effects: 1. It promotes cell proliferation through estroge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P1/16
Inventor 殷正丰刘鹏康晓燕陆怀秀
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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