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XAGE-1 gene and application of its expression production in tumour treatment and diagnosis

A technology for expressing XAGE-1 and its products, which can be used in gene therapy, application, anti-tumor drugs, etc., and can solve the problems of insufficient specificity and sensitivity

Inactive Publication Date: 2008-09-24
FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the preferred indicator is alpha-fetoprotein (alpha-fetoprotein, AFP), but its specificity and sensitivity have many shortcomings

Method used

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  • XAGE-1 gene and application of its expression production in tumour treatment and diagnosis
  • XAGE-1 gene and application of its expression production in tumour treatment and diagnosis
  • XAGE-1 gene and application of its expression production in tumour treatment and diagnosis

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Embodiment Construction

[0025] 1. Construction of eukaryotic plasmid and adenoviral vector expressing the gene: the XAGE-1b cDNA sequence was amplified from ACC-M cDNA by RT-PCR method, inserted into pEGFP-N1 plasmid (Clontech, Mountain View, USA) to obtain Eukaryotic expression vector of XAGE-1b: pE-Xb. And transfect ACC-2 cells (Shanghai Ninth People's Hospital Extraoral Tumor Laboratory), utilize G418 resistance to screen the stable cell lines of the vector: ACC2-Xb-19 (monoclonal strain), ACC2-Xb-M (multiple clones) clones).

[0026] The XAGE-1b cDNA sequence was amplified by PCR and inserted into the pAdTrack-CMV (Stragene, USA) plasmid. The recombinant plasmid was obtained after the homologous recombination of the shuttle plasmid and the backbone plasmid pAdEasy-1 (Stragene, USA) in the BJ5183 strain (Stragene, USA). The plasmid was transfected into 293T cells (ATCC, USA) and packaged to obtain mature virus particles: Adv-1b.

[0027] Both RT-PCR and Western-Blot proved that ACC-2 cells infec...

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Abstract

The invention belongs to the technical field of gene therapy and diagnosis, in particular relates to the application of a tumor antigen gene XAGE-1 and expression products thereof in tumor therapy and diagnosis. The vital function of XAGE-1 gene in the growth and the transfer of tumors is found for the first time herein, and XAGE-1 gene is an important symbol of tumors. According to the research, XAGE-1 can promote proliferation, transfer and invasion capabilities of cells of adenoid cystocarcinoma of salivary gland and various tumor cells. The gene and the expression products thereof can be used as drug targets to develop various tumor treatment drugs, in particular the drugs for resisting the growth and transfer of tumors. XAGE-1 and the expression products thereof can be used as the symbols of tumors for tumor diagnosis and analysis of illness, and for developing various diagnostic reagent kits and methods, thus significantly improving the diagnosis level of tumors, in particular incipient tumors. The specificity regulatory sequence of XAGE-1 can be applied in tumor therapy and so on according to the transcriptional regulatory mechanism of XAGE-1.

Description

technical field [0001] The invention belongs to the technical field of gene therapy and diagnosis, and specifically relates to the application of a new tumor antigen-XAGE-1 and its expression product in tumor treatment and diagnosis. Background technique [0002] Tumor-testis antigen (CT antigen) is a type of tumor antigen that is mainly expressed in normal germline reproductive tissues (especially immune-privileged testicular tissues) and cancerous tissues, and is not expressed at all or only slightly expressed in other normal tissues. Some well-known tumor antigens, such as MAGE, BAGE, GAGE, etc. belong to CT antigens. The precise and restricted distribution of CT antigens makes it a hotspot in the current study of tumor immunotherapy. XAGE / CT12 is a new class of genes discovered by bioinformatics methods, including XAGE-1, XAGE-2 and XAGE-3. Among them, the tumor expression profiles of XAGE-2 and XAGE-3 are very narrow, and there are few studies. In addition to the expr...

Claims

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Application Information

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IPC IPC(8): C12N15/12C12N15/63C12N15/861C12N5/10C07K14/435C12Q1/68G01N33/574A61K38/17A61K48/00A61P35/00
Inventor 朱乃硕刘洋宋玉亮
Owner FUDAN UNIV
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