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Method for preparing gellan gum by using waste glucose mother liquor

A technology of glucose mother liquor and gellan gum, which is applied in the field of microbial fermentation and extraction, can solve the problems of wasting food resources, not complying with industrial policies, and high manufacturing costs, and achieves the effects of superior compatibility, low manufacturing costs, and reduced production costs.

Active Publication Date: 2008-08-13
HEBEI HENBO BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned existing technologies have the disadvantages of wasting a large amount of food resources, high manufacturing costs, and not conforming to national industrial policies.

Method used

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Embodiment Construction

[0048] The present invention will be further described below in conjunction with the embodiments, but not as a limitation to the present invention.

[0049] A method for preparing gellan gum using waste glucose mother liquor, the method includes the following process steps:

[0050] A. The slant strain is expanded and cultivated into seed liquid;

[0051] B. Connect the seed liquid prepared in step A to the fermentation medium with an inoculum of 15% of the seed liquid: fermentation medium for fermentation, wherein the fermentation medium includes the following components by mass:

[0052] Waste glucose mother liquor 4% Soy protein powder 0.2% Enzymatic hydrolysis cottonseed protein 0.3%

[0053] Soy flour 0.2% Disodium hydrogen phosphate 0.15% Soybean oil 0.15%

[0054] Polyether defoamer 0.08%

[0055] The technological conditions in the fermentation process are:

[0056] Within 10 hours: temperature 31℃, pressure 0.04MPa, pH value 7.3, ventilation 0.3vvm;

[0057] 10-20 hours: ...

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Abstract

The invention belongs to tiny organism fermentation and abstraction, particularly relates to a method for preparing Wenlun glue prepared with exhausted molasses or deposed gluecose mother liquor. The preparing process comprises expanding and culturing oblique culture to prepare seed liquor, transferring the seed liquor into fermentation medium with deposed gluecose mother liquor as main carbon source, ventilation fermenting by assisting necessary nitrogen source and nutritive matter, pretreating, extracting, cleaning, drying and crushing to obtain Wenlun glue. The invention solves a series problems existing in present technology such as wasting food supplies resource, high cost and not conforming national industry policy. Also the invention satisfies the re-using of exhausted molasses or deposed gluecose mother liquor and solves the problem of serious pollution caused by abandoning the same, meanwhile, the prepared Wenlun glue has same property, simple art, low cost and the technology advancing direction is suitable for national industry policy comparing with the product prepared in traditional method.

Description

Technical field [0001] The invention belongs to microbial fermentation and extraction, and specifically is a method for preparing gellan gum by using waste glucose mother liquor. Background technique [0002] At present, the preparation of gellan gum is to inoculate the production strains in a sterile medium with starch as the main carbon source for fermentation. The fermentation process is to carry out aerobic fermentation under the specified temperature and pH conditions, and the selection of production strains is small. Sphingomonas paucimobilis (Sphingomonas paucimobilis), the final fermentation product is extracted to obtain gellan gum. The above-mentioned prior art has the disadvantages of wasting a large amount of food resources, high manufacturing cost, and not conforming to the country's industrial policy. Summary of the invention [0003] The purpose of the present invention is to provide a method for preparing gellan gum using industrial waste-waste glucose mother liq...

Claims

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Application Information

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IPC IPC(8): C12P19/04C08B37/00C12R1/01
Inventor 张禹张国佩张茶张少华
Owner HEBEI HENBO BIO TECH
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