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Short peptide for inhibiting growing of liver cancer cell and application thereof

A technology for inhibiting liver cancer and short peptides, which is applied in the field of short peptide series for inhibiting the growth of liver cancer cells, can solve the problems of patients suffering, chemotherapy drugs, bone marrow suppression, death, etc., and achieve the effect of solving serious injuries

Inactive Publication Date: 2008-08-06
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The most common side effect of chemotherapy drugs is the extreme suppression of the patient's bone marrow, causing great pain to the patient, and even death
So far, in addition to early detection of cancer and surgical resection of tumor mass, there is still no specific drug to treat tumors that have spread [Cancer Biology, written by R.J.B Jin, translated by Liu Yixun, Science Press, 2002], Most of the existing clinically used drugs for cancer treatment are chemical compounds, while the application of short peptides in the direct treatment of cancer has not been reported in the literature

Method used

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  • Short peptide for inhibiting growing of liver cancer cell and application thereof
  • Short peptide for inhibiting growing of liver cancer cell and application thereof
  • Short peptide for inhibiting growing of liver cancer cell and application thereof

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Experimental program
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preparation example Construction

[0016] The preparation method of the short peptide adopts a chemical synthesis method, that is, a very mature solid-phase peptide synthesis method well known in the art, and either the Boc method or the Fmoc method can be used. The specific method is to couple the protected amino acids to the inert solid phase carrier one by one, and then use strong acid to cleave the peptide chain from the carrier, and remove the side chain protection at the same time.

[0017] The amino acid main sequence of the short peptide is a main sequence with fixed amino acid positions, and the corresponding amino acids are selected and arranged at the interval positions. For example, there are 3 kinds of Pro, Asn or His amino acids at the J position, and these 3 kinds of amino acids can be placed in a single place. At the position of J in , no matter which one is selected, the short peptide still has its original biological activity, that is, inhibiting the growth of malignant liver tumor cells, and t...

Embodiment 1

[0033] Test of inhibiting liver cancer cells (synthesis of the short peptide involved in the present invention: Pro-Val-Ser-Trp-Leu-Arg)

[0034] The human liver cancer cell line Hepg2 cells were cultured with RPMI 1640 medium containing 10% newborn bovine serum, with 5×10 5 The cell density per well was planted into a 96-well cell plate. After 24 hours, the short peptides involved in the present invention were added to the cell culture plate according to different concentrations, and an equal volume of PBS was used as the control group. After 24, 48, and 72 hours, The survival rate of living cells was tested by MTT method, and the inhibition rate of liver cancer cells was up to 71% compared with the control group.

Embodiment 2

[0036] Test of inhibiting liver cancer cells (synthesis of the short peptide involved in the present invention: Pro-Val-Serp-Trp-Leu-Arg)

[0037] Human liver cancer cell line Bel-7402 cells were cultured with RPMI 1640 medium containing 10% newborn bovine serum, with 5×10 5 The cell density per well was planted into a 96-well cell plate. After 24 hours, the short peptides involved in the present invention were added to the cell culture plate according to different concentrations, and an equal volume of PBS was used as the control group. After 24, 48, and 72 hours, The survival rate of living cells is tested by MTT method, and the inhibition rate of liver cancer cells can reach 75% when compared with the control group.

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Abstract

The invention provides a short peptide series which can inhibit the growth of hepatoma cell and an relative application, wherein the amino acid main series of the short peptide, analogue, derivative and modification have the resemblance of at least 70% and similarity of at least 90% with the amino acid main series of the short peptide, the short peptide, nucleic acid, short peptide section, short peptide analogue, short peptide derivative and short pepetide modification can be used to prepare the drug for treating the liver cancer with hepatoma cell increment, diagnosing and detecting liver caner occur and liver caner grade. The invention can resolve the defect of prior liver cancer drug which will damage normal cells as well as inhibit or kill hepatoma cell.

Description

technical field [0001] The invention belongs to the field of biotechnology and medicine, and more specifically relates to a series of short peptides for inhibiting the growth of liver cancer cells and applications thereof. Background technique [0002] Chemotherapy has always been the main part in the process of cancer occurrence and treatment. The existing clinical chemotherapeutic drugs, such as cyclophosphamide, cisplatin, cytarabine, doxorubicin and vincristine, etc., are mainly used to destroy the cell cycle, and RNA, DNA and protein are all of them. target of drugs. These chemotherapeutic drugs have their own toxicity. Whether one chemotherapeutic drug is used alone or several chemotherapeutic drugs are used in combination in clinical treatment, it has obvious curative effect on cancer and kills cancer cells. damage the patient's normal cells. The most common side effect of chemotherapy drugs is the extreme suppression of the patient's bone marrow, causing great pai...

Claims

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Application Information

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IPC IPC(8): C07K7/06C12N15/11C12Q1/68A61K38/08A61K31/7088A61K48/00A61P35/00G01N33/68
Inventor 李昊饶平凡
Owner FUZHOU UNIV
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