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Pipless fructus momordicae and culture method thereof

A cultivation method and technique of Luo Han Guo are applied in the directions of botanical equipment and methods, cultivation, plant genetic improvement, etc., to achieve the effects of simple and easy operation, high content and reduced production cost

Active Publication Date: 2010-10-27
桂林亦元生现代生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, seedless Luo Han Guo and its cultivation methods are still a blank in the world

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1) Cultivate many diploid female and male plant seedlings of Luo Han Guo Qingpi fruit, and plant them according to conventional techniques;

[0024] 2) When the male plants are budding, they are placed in an environment of 5°C to 60°C for variable temperature cultivation every day. Specifically, they are first cultivated at 5°C to 10°C for 8 hours, then placed at 15°C to 40°C for 4 hours, and then Place at 40°C-60°C for 4 hours, and finally place at 15°C-40°C for 8 hours to induce the formation of diploid large pollen;

[0025] 3) When the male plant blooms, the pollen is taken out, and the diploid large pollen is screened out therefrom;

[0026] 4) Put the diploid large pollen into the pollen germination solution, stir gently to make a paste, and hybridize with the haploid egg cells of the selected diploid female plant, and obtain hybrid seeds after the female plant bears fruit;

[0027] 5) Breeding the hybrid seeds into seedlings, taking the shoot tip meristems to de...

Embodiment 2

[0030] 1) Cultivate a plurality of grosvenoria grosvenori diploid female and male plant seedlings, and plant them according to conventional techniques;

[0031] 2) When the budding of the male plant is in the meiotic stage, inject pentoxychlor with a molar concentration of 300 μmol / L into the flower bud to induce the formation of diploid large pollen;

[0032] 3) When the male plant blooms, the pollen is taken out, and the diploid large pollen is screened out therefrom;

[0033] 4) Put the diploid large pollen into the pollen germination solution, stir gently to make a paste, hybridize with the haploid egg cells of the selected diploid female plant, and obtain hybrid seeds after the female plant bears fruit;

[0034] 5) Breeding the hybrid seeds into seedlings, taking the shoot tip meristems to detect the number of chromosomes, and screening out triploid plants;

[0035] 6) Planting triploid plants and diploid male plants according to conventional techniques, using diploid ma...

Embodiment 3

[0037] 1) Cultivate a plurality of mangosteen grosvenori diploid female and male plant seedlings, and plant them according to conventional techniques;

[0038] 2) When the budding of the male plant is in the meiotic stage, wrap the whole flower bud with a material with good water retention, drip a colchicine solution with a mass concentration of 0.05% on the water retention material for infiltration treatment, remove the wrapping material after 5 days, and induce Formation of diploid macropollen.

[0039] 3) When the male plant blooms, the pollen is taken out, and the diploid large pollen is screened out therefrom;

[0040] 4) Put the diploid large pollen into the pollen germination solution, stir gently to make a paste, hybridize with the haploid egg cells of the selected diploid female plant, and obtain hybrid seeds after the female plant bears fruit;

[0041] 5) Breeding the hybrid seeds into seedlings, taking the root tip meristems to detect the number of chromosomes, and...

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PUM

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Abstract

The invention discloses a seedless grosvenor momordica with high mogroside content, high whole fruit utilization rate and good taste, and a cultivating method for the seedless grosvenor momordica. The steps of the method are as follows: 1) cultivating and planting the diploid female and male seedlings of the grosvenor momordica; 2) when a male plant emerges a flower bud, inducing the formation of diploid big pollen; 3) picking the male flower that is induced to bloom and screen a diploid big pollen therein; 4) hybridizing the haploid oocyte of the diploid female plant after treating the diploid big pollen in the pollen germination solution, acquire the hybrid seeds after the female plant fruits; 5) propagating the hybrid seed into whole plant and then screening a triploid plant with the Chromosome number inspection; 6) planting the triploid plant and the diploid male plant, and carrying out artificial pollination to the male triploid plant with the male diploid plant when the plant is blooming, thus acquiring the seedless grosvenor momordica when the female plant fruits.

Description

(1) Technical field: [0001] The invention relates to the field of biotechnology breeding, in particular to seedless Luo Han Guo, and also relates to a cultivation method of the seedless Luo Han Guo. (two) background technology: [0002] Luo Han Guo Siraitia grosvenororii (Swingle) C. Jeffrey is the fruit of the perennial vine of Cucurbitaceae Luo Han Guo. It is unique to my country and has a history of more than 300 years for both medicine and food. Modern research shows that the medicinal active ingredient of Luo Han Guo is mogroside, which has the functions of antitussive and expectorant, two-way regulation of intestinal motility, scavenging free radicals in the human body, inhibiting caries caused by Streptococcus mutans in the oral cavity, protecting the liver and preventing cancer, and lowering serum cholesterol , hypoglycemic, hypolipidemic effect. Mogroside is one of the strongest sweet substances in the world, among which glucoside V is 300 times sweeter than sucros...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04A01H3/00A01G17/00
Inventor 蒋向军孙仲序刘燕欢蒋剑刚楼佳鹏覃吉胜刘凤英黄争艳苏建春
Owner 桂林亦元生现代生物技术有限公司
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