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Photon crystal composite encoding microsphere and preparation method

A technology of photonic crystal microspheres and photonic crystals, which is applied in the direction of material analysis, measuring devices, instruments, etc. through optical means, can solve the problems of difficult to distinguish emission peaks of emitted light, complex detection equipment, complex preparation, etc., and achieve color adjustable , high photochemical stability and simple preparation method

Inactive Publication Date: 2008-07-09
SOUTHEAST UNIV
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AI Technical Summary

Problems solved by technology

The preparation of these encoding methods and encoding carriers is relatively complicated, and the detection equipment required for the decoding process is complex and costly
In addition, the use of fluorescent fuel coding also has problems such as light fading, the emission peaks of emitted light are difficult to distinguish, and biocompatibility.

Method used

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  • Photon crystal composite encoding microsphere and preparation method

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preparation example Construction

[0025] The preparation method comprises the following steps:

[0026] Select photonic crystal microspheres with different codes and perform surface treatment on them to obtain negatively charged photonic crystal coded microspheres;

[0027] Using the layer-by-layer adsorption method, the positive polyelectrolyte is first adsorbed on the surface of the photonic crystal microsphere, then the negative polyelectrolyte is adsorbed, and after the positive polyelectrolyte is adsorbed, the quantum dots with different characteristic emission peaks are adsorbed on the outer surface of the microsphere;

[0028] To adsorb quantum dots with different layers or different sizes, the layers are separated by three layers of polyelectrolyte;

[0029] The quantum dots on the outermost layer continue to adsorb four layers of polyelectrolytes for easy detection.

[0030] At the same time, quantum dots of the same type but different sizes are used to mark the substance to be detected, and the conc...

Embodiment 1

[0031] Embodiment one. Preparation of water-soluble CdTe-labeled polystyrene microspheres

[0032]1 Select polystyrene microspheres coded at 600nm and 540nm respectively. Since the synthesized polystyrene particles have carboxyl groups, the surface of the prepared microspheres also has negative charges.

[0033] 2 Soak the prepared microspheres in 0.5mol / L NaCl solution of 1mg / mL PAH first, let it stand for adsorption for 20 minutes, and rinse with ultrapure water three times to wash off excess PAH. Then soak the microspheres in 0.5 mol / L NaCl solution of 1 mg / mL PSS, and also stand still for adsorption and centrifugal washing. After another layer of PAH was adsorbed, the microspheres were soaked in thioglycolic acid-modified CdTe 540 (The emission spectrum is at 540nm) in an aqueous solution, let stand for adsorption for 60 minutes, and then centrifuge and wash.

[0034] 3Repeat the above steps. After adsorbing three layers of polyelectrolyte, the second layer of CdTe is ...

Embodiment 2

[0036] Embodiment two. Preparation of water-soluble CdTe-labeled silica microspheres

[0037] 1 Select 400nm, 460nm and 540nm coded silica microspheres respectively, and immerse them in 70% (V / V0) sulfuric acid and 30% hydrogen peroxide solution overnight, so that the surface of the prepared microspheres is negatively charged.

[0038] 2 Soak the prepared microspheres in 0.5mol / L NaCl solution of 1mg / mL PAH first, let it stand for adsorption for 20 minutes, and rinse with ultrapure water three times to wash off excess PAH. Then soak the microspheres in 0.5 mol / L NaCl solution of 1 mg / mL PSS, and also stand still for adsorption and centrifugal washing. After another layer of PAH was adsorbed, the microspheres were soaked in thioglycolic acid-modified CdTe 540 (The emission spectrum is at 540nm) in an aqueous solution, let stand for adsorption for 60 minutes, and then centrifuge and wash.

[0039] 3Repeat the above steps. After adsorbing three layers of polyelectrolyte, the ...

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Abstract

A photonic crystal compound-coding microsphere and preparation method relates to a method for compound-coding microsphere from a specific light reflection peak of photonic crystal as well as characteristic emission peak of quantum dots adsorbed by an outer layer and the intensity of the characteristic emission peak. The method is characterized in that the microsphere is made of colloid particles; quantum dots wrap the surface of the photonic crystal microsphere layer by layer; and quantum dot coding can be integrated with photonic crystal microsphere coding by wraping different quantum dots and different layers of quantum dots, thus improving the coding quantity. The polymer microsphere has the advantages of good coding stability, easy control, low production cost, simple operation, high efficiency, large detection flux, etc.

Description

technical field [0001] The invention relates to a method for composite encoding of microspheres by the specific light reflection peak of photonic crystals, the characteristic emission peak and characteristic emission peak intensity of quantum dots adsorbed on the outer layer, and belongs to the technical fields of biomedical research, environmental monitoring and clinical detection. Background technique [0002] After the human genome sequence was decoded, the function of the gene and the interaction between the proteins encoded by the gene became a research hotspot, and human beings entered the post-genome era. A notable feature of the post-genome era is to study a large number of interactions between proteins and nucleic acids, proteins and proteins, and proteins and drugs. The study of these interactions is almost impossible with traditional experimental methods. Therefore, a high-throughput test platform is needed. The high-throughput test platform requires a high-throu...

Claims

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Application Information

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IPC IPC(8): G01N21/00
Inventor 顾忠泽李娟赵祥伟赵远锦扈靖
Owner SOUTHEAST UNIV
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