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Method for detecting endometriosis blood plasma marker protein

An endometrial and protein technology is used in the application of SELDI technology to detect plasma marker proteins of endometriosis in vitro and diagnose endometriosis. It can solve the problems of low molecular weight and low abundance proteins Detection, two-dimensional electrophoresis difficulties and other problems, to achieve the effect of improving the diagnosis rate

Inactive Publication Date: 2008-07-02
PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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Problems solved by technology

This technology overcomes many problems in traditional two-dimensional electrophoresis, such as membrane protein separation, strong polar proteins, low molecular weight and low abundance proteins are difficult to detect, etc. [1-3]
Especially for disease-associated secreted proteins in plasma, two-dimensional electrophoresis is more difficult

Method used

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  • Method for detecting endometriosis blood plasma marker protein
  • Method for detecting endometriosis blood plasma marker protein
  • Method for detecting endometriosis blood plasma marker protein

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Embodiment Construction

[0023] The present invention will now be further illustrated in conjunction with specific examples, and it should be understood that these examples are for illustration purposes only, rather than limiting the scope of the present invention.

[0024] Materials and methods

[0025] 1. Specimen collection and processing

[0026] The preoperative plasma of patients with endometriosis diagnosed during the operation was obtained from the Peking Union Medical College Hospital of the Chinese Academy of Medical Sciences, and all patients did not take any drugs before the operation. The healthy paired plasma with endometriosis control was obtained from the blood bank of Peking Union Medical College Hospital. Detailed clinical information is shown in the table below.

[0027] Table 3. Clinical data of patients with endometriosis and healthy women

[0028]

[0029] The peripheral blood of patients before operation and healthy volunteers was collected into test tubes containing 0.1 w...

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Abstract

The invention provides a method for in vitro detection of plasma protein marker of endometriosis, which comprises the following steps of: collecting plasma sample from a patient; detecting the protein in the sample with mass spectrograph, especially using SELDI-TOF-MS technique; comparing the obtained result with the result obtained from a normal person, and determining the plasma protein marker of endometriosis in combination of bioinformatics. The invention also relates to the use of the plasma protein marker obtained using the method and relates detection reagents in preparing products for diagnosis of endometriosis.

Description

technical field [0001] The invention relates to the field of diagnosis of endometriosis, in particular to a method for detecting plasma marker protein of endometriosis in vitro by using SELDI technology. The present invention also relates to a series of plasma proteins thus obtained that can be used as potential biomarkers of endometriosis and its application in diagnosing endometriosis. Background technique [0002] Endometriosis is a frequently occurring disease in women of childbearing age, causing a variety of clinical symptoms. In recent years, the incidence rate has shown an obvious upward trend, reaching 10%-15%, accounting for more than 30% of gynecological operations. Dysmenorrhea, lower abdominal pain and infertility caused by endometriosis seriously affect women's health and quality of life. Although endometriosis is a benign disease, its lesions are extensive, diverse, extremely invasive and recurrent, and the damage to organ structure and function is equivalen...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N30/00
Inventor 刘海元郎景和
Owner PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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