Tobacco cucumber mosaic virus field rapid detection test paper preparation method

Abstract

The invention discloses a preparation method of test strip for rapid field detection of tobacco cucumber mosaic virus, which comprises the following steps of: preparing an antibody, preparing colloidal gold, preparing a colloidal gold-antibody conjugate, preparing a nitrocellulose filter, and preparing a colloidal gold chromatographic test strip. The prepared test strip for rapid field detection of tobacco cucumber mosaic virus according to the technical proposal has an optimum detection temperature of 15-35DEG C, sample detection amount of 0.1-0.3g and tobacco sample area of 3-5cm<2>. The detection method comprises charging tobacco leaf in a plastic bag containing buffer, grinding, immersing the test strip in the buffer, reacting for 30min, and observing the result. The false positive rate is less than 1%. The invention has the advantages of convenient application and high reliability; and is especially suitable for rapid field detection.

Description

Technical field [0001] The invention belongs to detection materials, and particularly relates to a method for preparing a test strip for rapid field detection of tobacco-cucumber mosaic virus. Background technique [0002] Tobacco and cucumber mosaic disease occurs in all tobacco-producing areas in my country. In recent years, the damage has gradually become heavier than ordinary mosaic disease. It causes large economic losses every year in Henan, Shandong, Shaanxi, Sichuan, Heilongjiang, Liaoning and other provinces. One of the important viral diseases in our country. The nucleic acid of Cucumber mosaic virus (CMV) is a triad of linear positive-strand ssRNA, which is mainly overwintered on overwintering vegetables and perennial weeds. In the following spring, toxic winged aphids stabbing smoking leaves to form initial infections , The field is infested by aphids and friction contact. In the early stage of tobacco infection, only symptoms such as heart leaf veins, mosaic and othe...

AI Technical Summary

Problems solved by technology

[0004] However, both of these methods require the extraction and purification of viruses or nucleic acids in the samples, and equipment such as ultra-high-speed centrifuges and electron microscopes are required. Although the specificity of serological methods is relatively strong, when the virus concentration is low, the antiserum prepared is less effective. The price is not high, the detection effect is not ideal